Low-Frequency, Whole Body Vibration Induced Neurite Outgrowth by Pc12m3 Cells with Impaired Nerve Growth Factor-Induced Neurite Outgrowth
|Yoshihisa Koike1*, Reiko Tutida1, Yuko Hayashi1, Yoko Yamanishi1 and Yoshio Kano2|
|1Department of Occupational Therapy, Faculty of Health and Welfare, Prefectural University of Hiroshima, Mihara City, Hiroshima 723-005, Japan|
|2Department of Occupational Therapy, School of Health Science, Kibi international University, 8 Iga-machi Takahashi City, Okayama 716-8508, Japan|
|Corresponding Author :||Yoshihisa Koike
Department of Occupational Therapy
Faculty of Health and Welfare
Prefectural University of Hiroshima, Mihara City
Hiroshima 723-005, Japan
E-mail: [email protected]
|Received October 30, 2014; Accepted February 05, 2015; Published February 12, 2015|
|Citation: Koike Y, Tutida R, Hayashi Y, Yamanishi Y, Kano Y (2015) Low-Frequency, Whole Body Vibration Induced Neurite Outgrowth by Pc12m3 Cells with Impaired Nerve Growth Factor-Induced Neurite Outgrowth. J Nov Physiother 5:249. doi: 10.4172/2165-7025.1000249|
|Copyright: © 2015 Koike Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
Purpose: To investigate the effects of very low-frequency whole body vibration (WBV) stimulation using PC12 m3 cells.
Methods: Mutant, drug-hypersensitive, PC12 m3 cells were obtained by means of continuous culture of neural PC12 cells and then stimulated by low-frequency vibration at frequencies of 7–13 Hz for 5–20 min with simultaneous exposure to nerve growth factor (NGF).
Results: The frequency of neurite outgrowth by PC12 m3 cells induced by 7-Hz vibratory stimulation was approximately 3-fold greater than that induced by NGF alone. Moreover, activated cyclic-AMP responsive element (CRE)-binding protein (CREB) expression was induced in PC12 m3 cells stimulated by 7-Hz vibration.
Conclusion: Low-frequency vibratory stimulation induces neurite outgrowth via a p38 mitogen-activated protein kinase/CREB signaling pathway in PC12 m3 cells. Together, these results suggest that WBV is an efficient method to stimulate neurite growth.