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Mechanistic Insights of the Infiltrated Cells in Large Porous Scaffolds Based on Microscopic Analysis of the Cells in Novel Scale-Down Culture Systems | Abstract

International Journal of Microscopy
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Research Article

Mechanistic Insights of the Infiltrated Cells in Large Porous Scaffolds Based on Microscopic Analysis of the Cells in Novel Scale-Down Culture Systems

Ling Yu1, Cai MSc1, GuoXia Han1 and Tao Sun2*

1Department of Biological Sciences, Xi'an Jiaotong-Liverpool University, Suzhou, China

2Department of Chemical Engineering, Centre for Biological Engineering, Loughborough University, UK

*Corresponding Author:
Tao Sun
Department of Chemical Engineering
Centre for Biological Engineering
Loughborough University, UK
Tel: +44 (0)1509 223214
E-mail: [email protected]

Received date: September 04, 2017; Accepted date: September 12, 2017; Published date: September 20, 2017

Citation: Yu L, Cai MSc, Han G, Sun T (2017) Mechanistic Insights of the Infiltrated Cells in Large Porous Scaffolds Based on Microscopic Analysis of the Cells in Novel Scale-Down Culture Systems. Int J Microsc 1: 101.

Copyright: © 2017 Yu L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Objective: Investigation of the cells cultivated within large porous scaffolds is problematic. This study aimed to develop scale-down culture models (SDCMs) for high throughput microscopic analysis of the infiltrated cells. Methods: Due to the advantages of biocompatibility and transparency, commercial glass capillaries with different lengths (5-80mm) and inside diameters (0.3, 1.0, 1.5, 1.7, 2.0mm) were tailored and situated in petri-dishes to mimic the interconnected pore structures within thick porous scaffolds. For the ease of in situ cell assessment, both horizontal and vertical SDCMs were developed and integrated with conventional microscopic technologies. Results: Evaluation experiments using migratory human dermal fibroblasts indicated that these SDCMs were able to simulate varying microenvironments surrounding infiltrated cells within large scaffolds. As both individual and aggregated cells at different parts of the capillaries were imaged noninvasively with sufficient high resolutions, it was feasible to track the dynamic tissue formation processes without sacrificing the delicate samples. Conclusion: This research demonstrated that these SDCMs could rapidly provide reliable data for the mechanistic understanding of various overarching factors that contribute the colonization of the infiltrated cells in large porous scaffolds.

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