Microtuber Induction of Two Potato (Solanum tuberosum L.) Varieties | OMICS International | Abstract
ISSN: 2329-8863

Advances in Crop Science and Technology
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Research Article

Microtuber Induction of Two Potato (Solanum tuberosum L.) Varieties

Miheretu Fufa1* and Mulugeta Diro2
1Oromia Agricultural Research Institute, Adami Tullu Agricultural Research Center, Plant Biotechnology Team, P.O. Box 35, Zeway, Ethiopia
2Southern Agricultural Research Institute, Ethiopia
Corresponding Author : Miheretu Fufa
Oromia Agricultural Research Institute
Adami Tullu Agricultural Research Center
Plant Biotechnology Team
P.O. Box 35, Zeway, Ethiopia
Tel: +251 116 187 346
E-mail: [email protected]
Received November 19, 2013; Accepted February 27, 2014; Published March 01, 2014
Citation: Fufa M, Diro M (2014) Microtuber Induction of Two Potato (Solanum tuberosum L.) Varieties. Adv Crop Sci Tech 2:122. doi: 10.4172/2329-8863.1000122
Copyright: © 2014 Fufa M. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse, distribution, and reproduction in any medium, provided the original author and source are credited.
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Two potato varieties namely, ‘Hunde’ and ‘Ararsa’ were tested for their microtuber induction under five levels of sucrose (40, 60, 80, 100 and 120 gl-1) in completely randomized design with 2×5 factorial combinations. The objective was to determine optimum concentration of sucrose for microtuber induction. In both varieties, among the five concentrations of sucrose, MS medium supplemented with 60 gl-1 sucrose exhibited a better response than the other concentrations in mean values of microtuber number, diameter, and weight and was found optimum. Accordingly, this medium produced, after 42.57 ± 0.58 days of culture, an average value of (1.97 ± 0.02) microtuber number, (3.60 ± 0.04 mm) microtuber diameter, and (0.08 ± 0.002 g) weight of microtuber in the variety Ararsa. On the other hand, it gave, after 35.67 ± 0.58 days of culture, mean value of (2.90 ± 0.031) microtuber number, (2.95 ± 0.01 mm) microtuber diameter, and (0.06 ± 0.001 g) weight of microtuber in the variety Hunde.