miR-143 is Associated with ER-ÃÂ±36 Expression Gastric Carcinoma of Xenografted ModelZou Feng1, Zhang Shangkun1,2, Li Yan1, Chen Ying1 and Liu Lijiang1*
- Corresponding Author:
- Liu Lijiang
Department of Pathology and Pathophysiology
School of Medicine, Jianghan University, Wuhan, China
E-mail: [email protected]
Received date September 28, 2013; Accepted date November 11, 2013; Published date November 18, 2013
Citation: Feng Z, Shangkun Z, Yan L, Ying C, Lijiang L (2013) miR-143 is Associated with ER-a36 Expression Gastric Carcinoma of Xenografted Model. J Biomim Biomater Tissue Eng 18:117. doi:10.4172/1662-100X.1000117
Copyright: © 2013 Feng Z, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
We investigated the expression profile of microRNA (miRNA) in ER-α36-mediated transplantable tumours. SGC7901 cell lines of higher or lower ER-α36 expression were constructed with a lentivirus vector. Five million SGC7901 cells were injected into nude mice to form a gastric xenografted carcinoma in the subcutaneous tissue of nude mice. Observation was performed for 30 days. The weight and propagation of the tumours were determined. miRNA expression profiles of the three SGC7901 cell lines were analysed by miRNA microarray. miR-143 expression in transplantable tumours was determined by real-time PCR using TaqMan assay. The expression level of ER-α36 was proportional to the size and propagation of gastric xenografted carcinomas in nude mice. The expression level of miR-143 significantly decreased in highly expressing ER-α36 SGC7901 cells and significantly increased in lowly expressing ER-α36 SGC7901 cells. miR-143 expression results in transplantable tumours amongst nude mice were consistent with the miRNA microarray findings. The expression of miR-143 was suppressed by high ER-α36 expression. These findings suggest that deregulation of miR-143 plays an important role in the progression of ER-α36-mediated gastric xenografted carcinoma.