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Hindi Research Article
Plant Regeneration of Kenyan Cassava (Manihot Esculenta Crantz) Genotypes
| Mathew PieroNgugi1*, Oduor Richard Okoth1, Omwoyo Richard Ombori2, Njagi Joan Murugi3, Mgutu Allan Jalemba1and Cheruiyot Richard Chelule2 | |
| 1Department of Biochemistry and Biotechnology, Kenyatta University, P.O Box 437844-00100, Nairobi, Kenya | |
| 2Department of Plant and Microbial Sciences, Kenyatta University, P.O Box 437844-00100, Nairobi, Kenya | |
| 3Department of Environmental Health, Kenyatta University, P.O Box 437844-00100, Nairobi, Kenya | |
| Corresponding Author : | Dereje Shimelis Ethiopian Sugar Corporation, Research and Training Division Variety Development Directorate, Biotechnology Research Team Wonji Research Center, P. O. box 15 Wonji, Ethiopia Tel: 251934074616 E-mail: d.shimelis@yahoo.com. |
| Received: January 22, 2015; Accepted: August 10, 2015; Published:August 17, 2015 | |
| Citation: Ngugi MP, Okoth OR, Ombori OR, Murugi NJ, Jalemba MA, et al. (2015) Plant Regeneration of Kenyan Cassava (Manihot Esculenta Crantz) Genotypes. Adv Crop Sci Tech 3:183. doi:10.4172/2329-8863.1000183 | |
| Copyright: © 2015 Shimelis D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
| Related article at Pubmed, Scholar Google | |
Abstract
A reproducible regeneration system based on direct somatic embryogenesis is described for Kenya cassava lines. Cassava plants were regenerated at high frequency by inducing shoot primordial on explants derived from cotyledons of cassava somatic embryos. Various parameters were evaluated on their effects on callus induction, somatic embryogenesis, maturation and germination of somatic embryos as well as recovery of regenerated plantlets. Immature leaf lobes were used as explants for somatic embryogenesis. Three Kenyan cassava genotypes viz; Adhiambo Lera, Kibanda Meno and Serere along with a model cultivar, TMS 60444 were used this system. Remarkable regeneration frequencies were observed in all the evaluated genotypes with Adhiambo Lera showing the best responses. As a result, a highly efficient plant regeneration protocol via germination of somatic embryos was achieved. This system enriches the scope of in vitro regeneration protocols for cassava and is envisaged to be a reliable prerequisite to genetic transformation of African cassava genotypes.
