The Effects of Sucrose on in vitro Tuberization of Potato Cultivars | OMICS International | Abstract
ISSN: 2329-8863

Advances in Crop Science and Technology
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Research Article

The Effects of Sucrose on in vitro Tuberization of Potato Cultivars

Miheretu Fufa1* and Mulugeta Diro2
1Adami Tullu Agricultural Research Center; Plant Biotechnology Team, P.O. Box 35, Zeway, Ethiopia
2Cascape Project Coordinator, Addis Ababa, Ethiopia
Corresponding Author : Miheretu Fufa
Adami Tullu Agricultural Research Center
Plant Biotechnology Team
P.O. Box 35, Zeway, Ethiopia
E-mail: [email protected]
Received September 19, 2013; Accepted September 23, 2013; Published September 26, 2013
Citation: Fufa M, Diro M (2013) The Effects of Sucrose on in vitro Tuberization of Potato Cultivars. Adv Crop Sci Tech 1:114. doi:10.4172/2329-8863.1000114
Copyright: © 2013 2013 Fufa M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Two potato varieties namely, ‘Hunde’ and ‘Ararsa’ were tested for in vitro Tuberization response under five levels of sucrose (40, 60, 80, 100 and 120 gram liter-1) in completely randomized design with 2×5 factorial combinations. The objective was to determine optimum concentration of sucrose for in vitro Tuberization. In both varieties, among the five concentrations of sucrose, Murashige and Skoog (MS) medium supplemented with 60 gram liter-1 sucrose exhibited a better response than the other concentrations in mean values of microtuber number, diameter, and weight and was found optimum. Accordingly, this medium gave an average value of (1.97 ± 0.02) microtuber number, (3.60 ± 0.04 mm) microtuber diameter, and (0.08 ± 0.002 gram) weight of microtuberin variety ‘Ararsa’ after 42.57 ± 0.58 days of culture. On the other hand, it gave mean value of (2.90 ± 0.031) microtuber number, (2.95 ± 0.01mm) microtuber diameter, and (0.06 ± 0.001 gram) weight of microtuber in variety ‘Hunde’ after 35.67 ± 0.58 days of culture.