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YES as a Tool for Detecting Estrogenic Activity of Some Food Additives Compounds: E 104, E 122, E 124, E 132 and E 171 | OMICS International | Abstract
ISSN: 2157-7625

Journal of Ecosystem & Ecography
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Research Article

YES as a Tool for Detecting Estrogenic Activity of Some Food Additives Compounds: E 104, E 122, E 124, E 132 and E 171

Ingrid Bazin1*, Aziza Ibn Hadj Hassine1, Wissem Mnif2 and Catherine Gonzalez1

1Ecole des Mines d’Alès, LGEI center, 6 avenue de Clavière 30319 Alès Cedex, France

2Laboratory of Biochemistry, Institute of Biotechnology, Monastir, Tunisia

*Corresponding Author:
Ingrid Bazin
Ecole des Mines d’Alès, LGEI center
6 avenue de Clavière 30319 Alès Cedex, France
E-mail: [email protected]

Received date: May 27, 2013; Accepted date: June 25, 2013; Published date: June 27, 2013

Citation: Bazin I, Hassine AIH, Mnif W, Gonzalez C (2013) YES as a Tool for Detecting Estrogenic Activity of Some Food Additives Compounds: E 104, E 122, E 124, E 132 and E 171. J Ecosys Ecograph 3:128. doi:10.4172/2157-7625.1000128

Copyright: © 2013 Bazin I, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and and source are credited.

Abstract

The toxicity (mutagenic and carcinogenic effects) of food dyes (used like food additive) has been well documented. However the endocrine disrupting effects of these dyes have been poorly investigated. We studied five commercial food dyes for their agonistic estrogen activity and do a comparison with estrogenic activity of some paraben (other food additives compounds). Total estrogenic activities were measured using the Yeast Estrogen Screen bioassay (YES). The estrogenic potency of the food dyes was measured by dose-response curves and compared to a doseresponse curve of 17-β-estradiol (E2), (reference compound). Estrogen EC50 values have been calculated using standard linear regression methods. Among these compounds, three food dyes, indigotine (E 132), the monoazo dye Ponceau 4R (E 124) and the inorganic dye titanium dioxide (E 171), revealed weak estrogenic activity. Negative results were obtained for Quinoline Yellow (E 104).

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