Journal of Analytical & Bioanalytical Techniques
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Measurement of ionized calcium is more important than the measurement of total calcium in serum samples.
Equations for calculating the amount of ionized calcium have been determined using the levels of total calcium; however, these
are not accurate, and therefore we devised new equations.
Our equations were derived from equilibrium equations of complex formation and acid dissociation such that the
amount of ionized calcium could be determined from the observed level of total calcium, albumin, total protein and inorganic
phosphate in serum. The ionized calcium level was calculated in 67 serum samples from healthy subjects and 34 outpatients
previously identified with abnormal serum calcium levels.
The correlation coefficient and the equation for linear regression between our method (y) and the electrode method (x)
was 0.953 and y=0.97x, respectively, at pH 7.4 as a factor value of 0.21. The factor value was the basis of the difference of values
for the amount of calcium phosphorus compounds by the electrode method.
Our method of calculation proved to be as useful and accurate as the electrode method and therefore extremely
useful for clinical diagnoses.
Susumu Takano has completed his master?s degree from Kobe University. He is a researcher and specialist in the department of clinical chemistry.
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