A New Method For Effectively Labeling Thiols On Proteins, Living Cells And Tissues | 10282
Journal of Analytical & Bioanalytical Techniques
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Using N-(2-Aminoethyl) maleimide-cysteine (StBu) (Mal-Cys) as a medium, protein thiols were converted into N-terminal
cysteines. After a biocompatible condensation reaction between the N-terminal cysteine and fluorescent probe
2-cyanobenzothiazole-Gly-Gly-Gly-fluorescein isothiocyanate (CBT-GGG-FITC), a new fluorogenic structure luciferin-GGG-
FITC was obtained. The latter exhibits a 2-fold fluorescence emission increase compared to the precursor moiety. Thus, a new
method for more effective labeling of thiols on proteins was developed. With enhanced fluorescence imaging of thiols on proteins,
outer membranes of living cells, and endothelial cell layers of small arteries was successfully achieved.
Dongxin Zhang has completed his Bachelor of Science at the age of 22 years from University of Science and Technology of China. He is a first year
Ph.D. candidate at Okinawa Institute of Science and Technology Graduate University.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals