Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar


A Novel Spectrophotometric Approach For The Measurement Of Submicromolar Concentrations Of Nitrite And Nitrate In Biological Fluids | 6110
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

A Novel Spectrophotometric Approach for the Measurement of Submicromolar Concentrations of Nitrite and Nitrate in Biological Fluids

International Conference & Exihibition On Analytical and Bioanalytical Techniques - 2010

Jaffar Nourooz-Zadeh, Athie Hajirahimkhan and Ebrahim Eftekhar

ScientificTracks Abstracts: J Anal Bioanal Techniques

DOI: 10.4172/2155-9872.1000001

Nitric oxide (NO) is an important mediator. Alteration in NO production has been implicated in a number of clinical conditions including renal failure. NO has a short half-life and therefore it is difficult to directly assess its concentrations body fluids. Hence, nitrite and/or nitrate levels are assessed as surrogate biomarkers of total NO production in the human body. A variety of analytical methods (i.e. spectrophotometric, spectroflourometric, HPLC-coupled to spectrophotometric- or spectroflourometric detectors, HPLC-MS and GC-MS have been developed for the measurement of NO metabolites. Of these, Griess-reaction is the most widely employed. The aims of present study were to develop: 1) an improved protocol for the preparation of serum nitrite and total nitrite (sum of nitrite + nitrate) by generating a genuine blank for every individual sample; 2) optimizing the condition for protein removal by ZnSO 4 ; and 3) exploring the effect of residual Cd, Cu and Zn on signal detection following the Griess reaction. Recipient of first kidney [n=20] were recruited. Serum sample collection started collected at day one pre-transplantation and continued up to 2 months post- transplantation (i.e. 20 separate time points). Sample preparation involved nitrate reduction employing a mixture of Cd/Cu as catalyst, protein removal, performance of the Griess reaction and UV measurement at 545 nm. It was found that the medians for nitrite and total nitrite levels in the candidates for renal transplantation were 0.6 �?¼M and 1 �?¼M, respectively. Three days after kidney transplantation, the corresponding levels dropped to 0.3 �?¼M and 0.4 �?¼M and remained fairly unchanged during the course of investigation. In conclusion, this investigation has revealed that total nitrite concentration in patients with end stage renal failure is approximately 1 �?¼M which is markedly lower than those data previously by other investigators using similar techniques. Our data also implies that final spectrophotmetric measurement is influenced by the protocol for protein removal as well as the presence of residual Cd and Cu. These findings may explain the large differences in reported values for above-mentioned NO metabolites in health and disease.