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Analysis Of Aflatoxins In Nonalcoholic Beer Using Liquid-liquid Extraction And Ultra Performance LC-MS/MS | 10277
ISSN: 2155-9872
Journal of Analytical & Bioanalytical Techniques
Open Access
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A flatoxins AFB1, AFB2, AFG1, and AFG2 are toxic secondary metabolites produced by
Aspergillus flavus
and
Aspergillus
parasiticus
and posses a potential threat to food safety. In the present work, liquid?liquid extraction and ultraperformance
LC-MS/MS method has been applied for the determination of four naturally occurring aflatoxins AFB1, AFB2, AFG1, and AFG2
in nonalcoholic beer. Aflatoxins extraction from nonalcoholic beer was carried out using liquid?liquid extraction procedure. The
effects of solvent-types were studied to obtain maximum recovery of the target analytes with minimum contamination. Among
different solvents, the aflatoxins extraction was best achieved using ethyl acetate. The obtained recoveries were ranged from 85
to 96% with good quality parameters: LOD values between 0.001 and 0.003 ng/mL, linearity of the calibration curve (r
2
>0.999),
and repeatability (run-to-run) and reproducibility (day-to-day) precisions with RSDs lower than 5% (n=5) achieved at 0.50
ng/mL concentration. The optimized liquid?liquid extraction in combination with ultraperformance LC-MS/MS was applied
successfully to the analysis of AFB1, AFB2, AFG1, and AFG2 aflatoxins in 11 nonalcoholic beers and were detected up to 15.31
ng/L in some of the samples.
Biography
Zeid A Alothman has completed his Ph.D. from Oklahoma State University 2006 and joined as an Assistant pProfessor in King Saud University. He is
the director of Advanced Materials Research Chair in College of Science, KSU. He has published a few books and more than 50 papers in reputed
journals and serving as an editorial board member of various reputed journals
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