Journal of Analytical & Bioanalytical Techniques
Open Access

Abstract

Esterification of starch and synthesis of infant formula fat analogous was carried out to expand the usefulness of industrial applications. Lipase obtained from Rhizopus oryzae was used for esterification: modification of maize starch with oleic acid as a acyl donors. Reactions performed with oleic acid by liquid-state gave a degree of, substitution (DS) 46.06% (DS 0.48), respectively. Thermogravimetric analysis showed that onset of decomposition is at a higher temperature (above 600 o C) for modified starch than the unmodified starch (280 o C). Swelling power lowered for modified starch as esterification renders starch more hydrophobic, making it suitable for biomedical applications as materials for bone fixation and replacements, carriers for controlled release of drugs and bioactive agents. Another reaction of structured lipids containing palmitic acid, resembling human milk fat (HMF), was synthesized. Under these conditions, the contents of palmitic acid (PA) and PA at sn-2 position (sn-2 PA) were 29.7 and 62.8%, respectively, and other observed FAs were all within the range of FAs of HMF. It can be concluded that the potential use of the structured lipids produced in infant formulas provide energy source delivering a reduced calorie value for new born, term and preterm infants. Unlike chemical esterification, enzymatic esterification is eco-friendly and avoids the use of nasty solvents.

Biography

Vinod Kumar has been a student at University of Delhi South Campus. He is pursing Ph.D. in Microbiology, under the guidance of Prof. R. K. Saxena at Department of Microbiology. His main research subject is to develop an Eco friendly system for Synthesis of bioactive molecules by enzymatic method which important in industrial sector. He has published 6 papers in reputed journals and papers presented in National/International conferences.