Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar


Anti-cancer Efficacy Of Novel Phenolic Polymer From Symphytum Asperum And S.caucasicum (Boraginaceae) Against Androgendependent And -independent Human Prostate Cancer Cells | 6014
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 4454

Journal of Analytical & Bioanalytical Techniques received 4454 citations as per Google Scholar report

Journal of Analytical & Bioanalytical Techniques peer review process verified at publons
Indexed In
  • CAS Source Index (CASSI)
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Academic Journals Database
  • Open J Gate
  • Genamics JournalSeek
  • JournalTOCs
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Ulrich's Periodicals Directory
  • Electronic Journals Library
  • RefSeek
  • Directory of Research Journal Indexing (DRJI)
  • Hamdard University
  • OCLC- WorldCat
  • Scholarsteer
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Euro Pub
Share This Page

Anti-cancer efficacy of novel phenolic polymer from Symphytum asperum and S.caucasicum (Boraginaceae) against androgendependent and -independent human prostate cancer cells

2nd International Conference on Analytical & Bioanalytical Techniques

V. Barbakadze

ScientificTracks Abstracts: J Anal Bioanal Techniques

DOI: 10.4172/2155-9872.S1.02

High-molecular (>1000 kDa) preparations from the crude polysaccharides of Symphytum asperum and S.caucasicum roots were isolated by ultrafi ltration on membrane fi lters with cut-off values of 1000 kDa and the elucidation of their principal structural units was carried out. According to IR, 13 C NMR, 1 H NMR, 2D heteronuclear 1 H/ 13 C HSQC spectral data the main structural element of these preparations was found to be a regularly substituted polyoxyethylene, namely poly[oxy-1-carboxy-2-(3,4- dihydroxyphenyl)ethylene] or poly[3-(3,4-dihydroxyphenyl)glyceric acid] (PDPGA). Such phenolic biopolymer to our knowledge has not been known and has been identifi ed for the fi rst time. Th is compound represents a new class of natural polyethers with a residue of 3-(3,4-dihydroxyphenyl)glyceric acid as the repeating unit. PDPGA possesses anticomplementary, antioxidant and anti-infl ammatory activities. We examined the effi cacy of PDPGA of S.asperum (PDGPA-SA) and S.caucasicum (PDGPA-SC) in androgen-dependent (LNCaP) and -independent (22Rv1 and PC3) human prostate cancer (PCA) cells. PDPGA-SA treatment (100 mcg/ml for 48h) decreases the live cell number by 65, 64 and 35% and increases the cell death by 16, 8 and 12 folds in LNCaP, 22Rv1 and PC3 cells, respectively. Similarly, PDPGA-SC treatment (100 mcg/ml for 48h) decreased the live cell number by 87, 25 and 33% and increased the cell death by 19, 10 and 9 folds in LNCaP, 22Rv1 and PC3 cells, respectively. Overall, present study identifi ed the strong effi cacy of these novel phytochemicals against PCA cells, and suggested that their pre-clinical effi cacy studies in PCA models are needed in future to move them forward for possible translational potential in PCA patients.

1978 and 1999 has completed his Ph.D and his D.Sci. at the ages of 33 and 54 years from Zelinsky Instiute of Organic Chemistry, Moscow, Russia and Durmishidze Institute of Biochemistry and Biotechnology, Tbilisi, Georgia, respectively. 2006 up to date he is the head of laboratory of plant biopolymers at the Tbilisi State Medical University I.Kutateladze Institute of Pharmacochemistry. 1996 and 2002 he has been a visiting scientist at Utrecht University (faculty of pharmacy, department of pharmacognosy), The Netherlands, by Utrecht University Scholarship and NATO Scienti fi c Program, respectively. He has published more than 50 papers in reputed journals.