Bio-analytical Determination Of Clopidogrel And Pantoprazole By RP-HPLC Method In Rat Plasma: Application To Drug Interraction Study | 10213
Journal of Analytical & Bioanalytical Techniques
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A simple, sensitive, rapid, robust and reproducible method for the bioanalytical determination of clopidogrel and pantoprazole
in rat plasma was developed using reverse phase high performance liquid chromatographic method. The analysis was
performed on C
(250?4.6 mm, 5 μm) column with a mobile phase consisting of 0.03 M potassium dihydrogen ortho phosphate
buffer (pH 3), acetonitrile in the ratio of 40:60 (v/v) with a flow rate of 1.2 ml/min. The analyte was monitored with UV detector
at 240 nm. In the developed method pantaprazole elutes at retention time of 2.6 min and clopidogrel at 8.2 min. The proposed
method is having linearity in the concentration range 10-50 μg/ml for both clopidogrel and pantoprazole. The method was
validated with respect to system suitability, linearity, precision, limit of detection (LOD) and limit of quantification (LOQ),
accuracy (recovery), ruggedness, robustness, stability, forced degradation studies (specificity). The proposed method can be
readily utilized for determination of clopidogrel and pantoprazole in routine plasma samples.
Bio analytical method, Drug Interaction study, Pentoprazole and clopidogrel.
B. M. Gurupadayya is working as Professor in the department of Pharmaceutical Chemistry. He has completed his Ph.D. degree from Kuvempu
University, Shimoga and postdoctoral studies from Gulbarga University. He has published more than 60 papers in reputed journals and has been
serving as an editorial board member of repute journals like Journal of Saudi Chemical Society, International Journal of Health and Applied Science
(IJHAS), International Journal of Spectroscopy and Current Pharmaceutical Analysis.
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