alexa
Reach Us +44-2476101207
Cancer Cell Targeting Using Folic Acidconjugated Fl Uorescent CdSe/ CdS/ZnS-MPA And CdTe-MSA Quantum Dots | 5997
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Cancer cell targeting using folic acidconjugated fl uorescent CdSe/ CdS/ZnS-MPA and CdTe-MSA quantum dots

2nd International Conference on Analytical & Bioanalytical Techniques

Gurpal Singh, Manoj Gautam, Vivek Bansal, Udit Soni, Vikas Arora, Sameer Sapra and Harpal Singh

Accepted Abstracts: J Anal Bioanal Techniques

DOI: 10.4172/2155-9872.S1.05

Abstract
Hydrophobic core-shell CdSe/CdS/ZnS and CdTe-MSA (Mercaptosuccinic acid) quantum dots (QDs) were synthesized by successive ion layer adsorption and reaction (SILAR) technique and direct aqueous synthesis using thiol stabilizers respectively. Synthesized QDs (CdSe/CdS/ZnS) were made water dispersible by ligand exchange with 3-mercaptopropionic acid (MPA). Characterization of the QDs was done by UV-vis spectroscopy, spectrofl uorometry and transmission electron microscopy (TEM), Fourier Transform infrared spectroscopy (FTIR), Confocal laser scanning microscopy (CLSM) and atomic force microscopy (AFM). CdSe/CdS/ZnS-MPA QDs with a size of 7-8 nm and CdTe-MSA QDs with a size of 3-3.5 nm were conjugated with folic acid (FA) using various techniques for targeting human cancer cells expressing folate receptor. Breast cancer cell line (MCF-7) was used for the cellular uptake studies, cellular QDs quantifi cation and cytotoxicity studies. In vitro cellular uptake studies and cellular QDs (CdSe/CdS/ZnS-MPA) quantifi cation was investigated with CLSM and spectrofl uorometry which demonstrated higher internalization of the folate-conjugated QDs by MCF-7 cells compared with the free QDs. In this study we also investigated in-vivo toxicity of folated QDs (CdSe/CdS/ZnS-MPA) in BALBc mice. For toxicity evaluation of injected QDs sample, body weight, organ coeffi cient, complete blood count (CBC), biochemistry panel assay (AST, ALT and ALP), histology were determined. Th e QDs (CdSe/CdS/ZnS-MPA) concentration (500nM) used for this study is suitable for in-vivo imaging. Th ese experiments confi rms that FA conjugated QDs are specifi cally internalized by MCF-7 tumor cells and safe in concentration used for in-vivo studies, suggesting their potential utility as targeted fl uorescent imaging agent for early stage cancer detection.
Biography
Top