Journal of Analytical & Bioanalytical Techniques
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Efforts to replace native peroxidase with its low molecular weight alternatives have stimulated a search for peroxidase
mimetics. Herein we describe the oxidation of luminol with hydrogen peroxide catalyzed by commercial available Fe
TAML activator 1a, which was showed to be more active catalyst than hemin. At Fe
-TAML activator 1a use in chemiluminescent
assay for H
determination the limit value (3σ) was 5x10-8 M, whereas in the presence of hemin the detection limit was
significantly higher and equal to 6x10
M. The linear ranges (
=0.98) of the assay were 6x10
M and 6x10
for FeIII-TAML 1a and hemin, respectively. The CV values for Fe
-TAML 1a-based assay measured within the working
range varied from 1.0 to 3.7% (n=4), whereas in the case of hemin -5.0 to 9.7% (n=4). Moreover, the sensitivity of Fe
1a-based method was 56 times higher than that of hemin-based method. The obtained results open good perspectives to apply
-TAML activator 1a in CL analytical methods instead of hemin, traditionally used peroxidase mimetic.
Marina M Vdovenko is a Scientific Researcher at the Department of Chemistry, Lomonosov Moscow State University (Russia). She has graduated with PhD degree in Biotechnology in 2011 under Prof. Ivan Yu Sakharov. Presently her work focuses on the development of novel sensitive chemiluminescent methods for their use in analytical practice. She has published more than 15 papers in peer-reviewed journals.
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