Coelenterazine-dependent Bioluminescent Proteins As Effective Reporters For In Vitro Assay | 18671
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 3463

Journal of Analytical & Bioanalytical Techniques received 3463 citations as per Google Scholar report

Journal of Analytical & Bioanalytical Techniques peer review process verified at publons
Indexed In
  • CAS Source Index (CASSI)
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Academic Journals Database
  • Open J Gate
  • Genamics JournalSeek
  • JournalTOCs
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Ulrich's Periodicals Directory
  • Electronic Journals Library
  • RefSeek
  • Directory of Research Journal Indexing (DRJI)
  • Hamdard University
  • OCLC- WorldCat
  • Scholarsteer
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Euro Pub
Share This Page

Coelenterazine-dependent bioluminescent proteins as effective reporters for in vitro assay

5th International Conference and Exhibition on Analytical & Bioanalytical Techniques

L A Frank

ScientificTracks Abstracts: J Anal Bioanal Tech

DOI: 10.4172/2155-9872.S1.017

Nowadays, the light-emitting proteins are the promising analytical tool for both in vitro and in vivo applications to meet the growing demands of science and medicine. The great part of analytical techniques is based on coelenterazine-dependent bioluminescent systems derived from luminous marine organisms. Bioluminescent signal in the organisms arises as a result of coelenterazine (CE) oxidation catalyzed by special enzymes, luciferases. The CE-dependent luciferases of different origin (coelenterates, ctenophores, copepods, ostracods, etc.) known for today are relatively small single-chain proteins having nothing common but a substrate. Luciferases of special type, Ca 2+ -regulated photoproteins, are stable complexes of apophotoprotein and pre-oxidized substrate molecule - peroxycoelenterazine, which is strongly but non-covalently immobilized in the protein hydrophobic cavity. Bioluminescent reaction is triggered with Ca 2+ producing coelenteramide, CO 2 , and a flash of blue light. Several CE-dependent luciferases are comprehensively studied as to biochemical properties, tertiary structures, bioluminescence mechanism etc. Application of photoproteins and luciferases as reporters in binding assay has many prospects due to the high quantum yield of bioluminescent reaction, providing high-sensitivity detection; assay robustness, reproducibility, and safety. Of special interest is the use of luciferases genetically modified so to obtain novel enzymes with unique properties such as shifted bioluminescence spectra, varied kinetics, thermostability, or the enzymes fused with polypeptide modules (mini antibodies, biotinylated site, etc.) that are responsible for the assay specificity.

L A Frank got her PhD degree in Biophysics (1997) and Doctor. of Sciences degree in Biology (2010) at the Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk. At present, she is a leading researcher of the Institute and Professor of the Siberian Federal University. Her research interests are concerned with investigating structure and function of light-emitting proteins and development of analytical systems on their base. She is the author and co-author of more than 100 publications and several international and Russian patents.