alexa Coelenterazine-dependent Bioluminescent Proteins As Effective Reporters For In Vitro Assay
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

5th International Conference and Exhibition on Analytical & Bioanalytical Techniques
August 18-20, 2014 DoubleTree by Hilton Beijing, China

L A Frank
ScientificTracks Abstracts: J Anal Bioanal Tech
DOI: 10.4172/2155-9872.S1.017
Abstract
Nowadays, the light-emitting proteins are the promising analytical tool for both in vitro and in vivo applications to meet the growing demands of science and medicine. The great part of analytical techniques is based on coelenterazine-dependent bioluminescent systems derived from luminous marine organisms. Bioluminescent signal in the organisms arises as a result of coelenterazine (CE) oxidation catalyzed by special enzymes, luciferases. The CE-dependent luciferases of different origin (coelenterates, ctenophores, copepods, ostracods, etc.) known for today are relatively small single-chain proteins having nothing common but a substrate. Luciferases of special type, Ca 2+ -regulated photoproteins, are stable complexes of apophotoprotein and pre-oxidized substrate molecule - peroxycoelenterazine, which is strongly but non-covalently immobilized in the protein hydrophobic cavity. Bioluminescent reaction is triggered with Ca 2+ producing coelenteramide, CO 2 , and a flash of blue light. Several CE-dependent luciferases are comprehensively studied as to biochemical properties, tertiary structures, bioluminescence mechanism etc. Application of photoproteins and luciferases as reporters in binding assay has many prospects due to the high quantum yield of bioluminescent reaction, providing high-sensitivity detection; assay robustness, reproducibility, and safety. Of special interest is the use of luciferases genetically modified so to obtain novel enzymes with unique properties such as shifted bioluminescence spectra, varied kinetics, thermostability, or the enzymes fused with polypeptide modules (mini antibodies, biotinylated site, etc.) that are responsible for the assay specificity.
Biography

L A Frank got her PhD degree in Biophysics (1997) and Doctor. of Sciences degree in Biology (2010) at the Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk. At present, she is a leading researcher of the Institute and Professor of the Siberian Federal University. Her research interests are concerned with investigating structure and function of light-emitting proteins and development of analytical systems on their base. She is the author and co-author of more than 100 publications and several international and Russian patents.

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords