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Comparative Study Of Twelve Functionalized Nanoparticle Reinforced Sol-gel For Microextraction Of Selected Drugs From Water Samples | 5984
Journal of Analytical & Bioanalytical Techniques
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A rapid and simple high performance thin layer chromatography method was developed and validated for simultaneous
estimation of Lafutidine and Domperidone from capsule dosage form. HPTLC separation was performed on silica gel
precoated aluminum plate 60 F
, (10 ? 10 cm) as stationary phase and ethyl acetate: methanol: ammonia (10:1:1v/v/v) as
mobile phase. The Densitometric scanning was performed at 265 nm. The R
values were found to be 0.59?0.02 and 0.47?0.02
for Lafutidine and Domperidone respectively. The method was validated for linearity, accuracy, precision and robustness. The
calibration plot was linear over the ranges 200-1200 ng /μl (r
= 0.9993? 0.016) for Lafutidine and 600-3600 ng/ μl (r
0.014) for Domperidone, respectively.The Limit of Detection and Limit of Quantitation were found to be 5.82 and 17.65 ng /μl for
Lafudine and 10.23 and 31.00 ng /μl for Domperidone, respectively. The average percentage recoveries were found to be 100.13 %
and 98.94% for Lafutidine and Domperidone respectively. Lafutidine and Domperidone were assayed and the content was found
to be 99.83?0.80 % and 99.26?0.65 % in capsule dosage form respectively.The present work established an accurate and rapid
validated HPTLC method for the simultaneous estimation of Lafutidine and Domperidone.
Dipmala Wagh has completed her B. Pharm at the age of 22 years from Pune University in 2010. She has published one research article in RJPR.
Currently, She is in M. Pharm second year and doing work on stability indicating study of drug as a research work in Sinhgad Research Center, Pune.
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