Journal of Analytical & Bioanalytical Techniques
Open Access

Abstract

Oxidized products of 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (PAPC) are pro-inflammatory and bioactive.The amounts and structures of oxidized PAPC products on lipoproteinshave not been fully understoodyet. In this study, lipoproteins were oxidized by Fenton reaction. Phospholipids on lipoproteins were then isolated by liquid-liquid and solid-phase extractions.ESI-MS analysiswas used to analyze oxidized PAPC products on human low-density lipoprotein (LDL) and high-density lipoprotein (HDL).A total of 9 long-chain and 10 short-chain oxidized PAPC products have beenanalyzed. The ion intensities of six oxidized PAPC products including m/z = 820.5, 836.5, 850.5, 622, 650 and 690 are significantly higher for oxidized LDL than for native LDL. However, the ion intensities of oxidized PAPC productsisolated fromoxidized HDL and native HDL are similar.

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