Conducting Polyamic Acid Membranes For Sensing And Site-directed Immobilization Of Proteins | 10267
Journal of Analytical & Bioanalytical Techniques
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Poly (amic) acid (PAA) is a functionalized conducting polymer substrate that provides electrochemical detection control
of biospecific binding. In this work, we report a biosensor platform based on the PAA for oriented immobilization of
biomolecules. The PAA was used to covalently attach biomolecules, resulting in a significant improvement in the detection
sensitivity. The biosensor sensing elements comprise a layer of PAA antibody (or antigen) composite self assembled onto gold (Au)
electrode via N-hydroxysuccinimide (NHS) and 1 ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) linking. The modified
PAA was characterized by Fourier transform infrared (FTIR), 1H nuclear magnetic resonance (NMR), and electrochemical
techniques. Cyclic voltammetry and impedance spectroscopy experiments conducted on electrodeposited PAA on Au electrode
using ferricyanide produced a measurable decrease in the diffusion coefficient compared with the bare electrode, indicating some
retardation of electron transfer within the bulk material of the PAA. Thereafter, the modified PAA surface was used to immobilize
antibodies and then to detect inducible nitric oxide synthase (a pain biomarker) and mouse immunoglobulin G (IgG) using
enzyme-linked immunosorbent assay (ELISA), surface plasmon resonance (SPR), and amperometric techniques. ELISA results
indicated a significant amplified signal by the modified PAA, whereas the SPR and amperometric biosensors produced significant
responses as the concentration of the antigen was increased.
Naumih M. Noah completed her Ph.D. from the State University of New York at Binghamton on May, 2012 where she studied New Concepts in Pain
Detection and Management using biochemical principles. She has published 8 papers from her Ph.D. work. She is currently lecturer of Analytical/
Bioanalytical chemistry at Kenyatta University in Kenya. She is interested in developing biosensors for rapid and sensitive detection of infectious and
neglected tropical diseases and integration of nanotechnology with health care in developing countries starting with Kenya.
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