alexa Design Of Nanoprobes For In Situ Analysis Of Cellular Functional Biomolecules | 18683
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

5th International Conference and Exhibition on Analytical & Bioanalytical Techniques

Huangxian Ju
ScientificTracks Abstracts: J Anal Bioanal Tech
DOI: 10.4172/2155-9872.S1.017
Abstract
Cellular functional biomolecules, such as glycans, microRNA, telomerase etc., regulate a variety of vital biological events, and thus have been regarded as attractive targets for biomedical research, molecular diagnostics and disease therapy. Due to the lack of noninvasive methods for interrogation of the related biological process, our recent efforts have been devoted to the fabrication of nanoprobes for in situ analysis of various cellular functional biomolecules and highly selective photodynamic therapy (PDT). For cell surface glycan detection, we proposed a novel competitive recognition format and a solution encoding strategy for quantitative analysis of multiple glycans on living cells, and fabricated a disposable electrochemical cytosensor array for simultaneous monitoring of multiple glycans on intact cell surfaces. Focusing on the urgent need for in situ analysis of intracellular telomerase, we designed a mesoporous silica nanoprobe and a nicked molecular beacon by using telomerase- responsive primer DNA to produce �off-on� imaging of intracellular telomerase activity. In order to monitor intracellular miRNA levels, a polyethylenimine-grafted graphene nanoribbon and a multifunctional SnO 2 nanoprobe were fabricated for simultaneous specific delivery, cell imaging and intracellular miRNA detection. The SnO 2 nanoprobe could also be used for regulating the expression of intracellular miRNA level. To achieve highly selective PDT, we proposed two cell-specific and acidic pH-activatable nanoparticles by introducing selenium into rubyrin core to enhance the 1O 2 generation efficiency and dimethylaminophenyl moiety at mesoposition of rubyrin to achieve pH-controllable activity, and using R16FP to generate 1O 2 and BDP-688 for therapeutic monitoring, which combined folate and selected aptamer at the surface of nanoparticles to obtain cancer cell targeting feature, respectively. We believe these studies would accelerate the understanding of biological roles of these functional biomolecules and provide valuable tools for in vivo clinical research.
Biography

Huangxian Ju received BS, MS and PhD from Nanjing University during 1982-1992 and was a postdoc in Montreal University from 1996-1997. He became an associate and full professor of Nanjing University in 1993 and 1999. He won the National Funds for National Distinguished Young Scholars in 2003 and National Creative Research Groups in 2006. He was selected as a Changjiang Professor in 2007, a Chief Scientist of National Basic Research Program of China in 2009 and the Director of State Key Laboratory of Analytical Chemistry for Life Science in 2011. His research interests focus on analytical biochemistry and molecular diagnosis. He has published 457 papers with an h-index of 60 and SCI citation of 12175, authored 27 patents, 2 English books, 6 Chinese books, and 6 Chinese and 8 English chapters.

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7