Journal of Analytical & Bioanalytical Techniques
Open Access

Abstract

Avalidated procedure was developed for the separation and clean-up of Organo-Chlorinated Pesticides (OCPs) extracted from fish samples, using different adsorbent materials and different solvents composition. Pressurized liquid extraction (PLE) has been utilized for the extraction of OCPs. PLE provides one of the best alternative to conventional extraction methods. The different adsorbent materials as well as solvents composition were evaluated for the best clean-up steps of OCPs extracted from fish samples. Among the eight examined adsorbent materials, florisil and alumina were selected with hexane: ethylacetate (8:2, v/v) solvent mixture throughout the experiment. The highest recoveries were obtained (75-99.8 %). Detection was performed by GC-MS in Negative Chemical Ionization (NCI) mode, due to its high sensitivity and selectivity to chlorinated compounds. Bio- Beads SX-3 Based on Gel permeation Chromatography (GPC) was utilized for the removal of fat. The accuracy of the method (n= 5) is expressed as recovery (%) that was calculated to be between 56% and 98%, and the precision of the method is expressed as Relative Standard Deviation (RSD), that was obtained to be between 5% and 28 %.

Biography

Amel Al-Rashdan has completed her PhD at the age of 31 years from University of Cincinnati, Ohio, USA. She is working in Kuwait Institute for scientific Research, she was a Department Manager for Central Analytical Laboratories for 5 years and now she is top management representative for ISO 9001:2008 and ISO 17025:2005. She has publications in reputed journals in the field of analytical chemistry in the hyphenated techniques such as HPLC-ICP-OES, HPLC-ICP-MS and GC-MS. She is also representing her country Kuwait and the institute as a member in Rotterdam Chemical Review Committee for Rotterdam Convention.