Development And Validation Of A RP- UFLC Method For Estimation Of Curcumin In Anti Diabetic Poly Herbal Formulation | 18770
Journal of Analytical & Bioanalytical Techniques
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Curcumin is an ancient medicine, having various remedial properties and it might be utilized for the medication of chronic
disorder like diabetes, cancer, arthritis, HIV infections and etc. The principle destination of our work was to develop a
simple, rapid and sensitive revers phase ultra fast liquid chromatographic (RP-UFLC) method for estimation of curcumin in
anti-diabetic poly herbal formulation (Mehagni). A basic chromatography partition was carried out by utilizing an reverse phase
column (250 x 4.6 mm) isocratic flow with the constituted a mixture of methanol and 2% acetic acid as a mobile phase at the
proportion of 70:30 v/v with the flow rate of l.2 ml/min. the absorbance measured at 420 nm by using UV spectrophotometry.
The method was validated as indicated by ICH guidelines distinctive parameters, such as specificity, sensitivity, linearity,
precision, accuracy, ruggedness, robustness and system suitability were performed. Optimized chromatographic conditions
were attained and effects demonstrated good peak resolution. The retention time was found at 5.02 min. The framework was
linear with a correlation co-efficient of 0.9945. %RSD of system and method precision were resolved to be 1.14 and 1.13. The
LOD & LOQ for curcumin was found to be 0.2 μg & 0.65 μg. The calibration curve was fixed at 5-25 μg/ml. The change in
analytical technique parameters, investigator and laboratory conditions did not influence the results.
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