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Development And Validation Of A UPLC Method For The Determination Of Praziquantel Residues On Pharmaceutical Manufacturing Equipment Surfaces | 10281
ISSN: 2155-9872
Journal of Analytical & Bioanalytical Techniques
Open Access
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In pharmaceutical industries, it is very important to remove drug residues from the equipment and areas used. The cleaning
procedure must be validated, so special attention must be devoted to the methods used for analysis of trace amounts of drugs.
A rapid, sensitive and specific reverse phase ultra performance liquid chromatographic (UPLC) method was developed for the
quantitative determination of praziquantel in cleaning validation swab samples. The method was validated using an ACQUITY
HSS C
18
, 50x2.1 mm, 1.8 μ column with a isocratic mobile phase containing a mixture of 1.36 g of potassium dihydrogenphosphate
in 1000 mL MilliQ water, 2 mL of triethylamine and pH adjusted to 2.3?0.05 with ortho-phosphoric acid, acetonitrile and
methanol (50:40:10 v/v). The flow rate of the mobile phase was 0.5 mL min
-1
with a column temperature of 35?C and detection
wavelength at 210 nm using PDA detector. The injection volume was 2 μl. Cotton swabs, moisten with acetonitrile were used to
remove any residue of drug from stainless steel, teflon, rubber and silicon plates which mimic the production equipment surface
and the mean extraction-recovery was found to be 91.8. The selected chromatographic condition was found to effectively elute
praziquantel with retention time of 1.37 min. The proposed method was found to be linear over the range of 0.3 to 150 μg/mL
and correlation coefficient obtained is 0.9998. The proposed method was found to be accurate, precise, reproducible and specific
and it can also be used for routine quality control analysis of these drugs in biological samples either alone or in combined
pharmaceutical dosage forms.
Keywords:
Cleaning validation, Praziquantel, Residues, Swab analysis, UPLC.
Biography
R. S. Chandan is perusing his Ph.D. in Dr. M. G. R. University, Maduravoyal, Chennai under the guidance of Dr. M. Vasudevan, Professor, Department
of Industrial Biotechnology, Dr. M. G. R. University, Maduravoyal, Chennai. He has published 14 papers in reputed journals.
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