Development And Validation Of HPTLC Method For An Anticold Formulation | 5890
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

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Development and validation of HPTLC method for an anticold formulation

3rd International Conference and Exhibition on Analytical & Bioanalytical Techniques

Gouri G. Joshi

Posters: J Anal Bioanal Techniques

DOI: 10.4172/2155-9872.S1.010

Advancements in the hybrid Qq-TOF instruments provide the ability to perform targeted and non-targeted multi-residue screening and Quantitation on a routine basis. Such instruments provide high resolution, accurate mass MS and MS/MS information enabling high quality screening for virtually every known chemical, identify and quantify undesirable contaminants using MRMHR quickly and easily. Resolution > 20,000 (at full width half height) and mass accuracy <5 ppm is often sufficient to separate the analytes of interest from interfering matrices and, thus, is a requirement for compound identification in various guidelines (EU Commission Decision #2002/657/EC; SANCO Document: #SANCO/10684/2009). High resolution MS and MS/ MS data allows generating very narrow XIC window of 10 to 20mDa for both parent as well as product ions. This capability provides adds an additional level of selectivity for any chemical residues in complex samples for targeted analysis workflows resulting in the best Signal-to-Noise ratio. High confidence in results will be based upon retention times, accurate mass, isotopic pattern and MS/MS library searching. This presentation will cover a) the unique HRMS workflows for multi-residue screening, b) the benefits of MRMHR for routine high resolution Quantitation for food contaminants.
Praveen K. Sharma received his Ph.D, degree from University of Delhi and Postgraduate diploma in Intellectual Property Rights from ILI, New Delhi. He has published more than 11 research papers in peer-reviewed journals in the area of identification of secondary metabolites and their biological efficacies. He is currently working as Application scientist for AB SCIEX India and is responsible for Applied Markets and Clinical Research support activities in India.