Development And Validation Of RP-HPLC Method For Simultaneous Estimation Of Thiocolchicoside And Dexketoprofen Trometemol In Combined Dosage Form | 10280
Journal of Analytical & Bioanalytical Techniques
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A simple, precise and rapid reverse-phase HPLC method has been developed and subsequently validated for the simultaneous
estimation of thiocolchicoside and dexketoprofen trometemol from their combination drug product. The proposed RP-
HPLC method utilizes a Oyster BDS C
, 5 μm, 250 mm ? 4.6 mm i.d. column, at ambient temperature; optimum mobile phase
consisted of methanol:ammonium dihygrogen phosphate buffer (55:45, v/v), pH adjusted 7.3 with triethyl amine; effluent flow
rate monitored at 1.5 mL min-1, and detection at 290 nm with UV-Visible detector. The described method was linear over
the range of 10-60 μg/ml and 12.5-75 μg/ml with co-efficient of correlation, (r
)=0.998 and (r
)=0.997 for thiocolchicoside and
dexketoprofen trometemol, respectively. The mean recovery was found to be 100.51?1.30% and 99.69?0.86% for thiocolchicoside
and dexketoprofen trometemol, respectively. The intermediate precision data obtained under different experimental setup, the
calculated value of coefficient of variation (CV, %) was found to be less than critical value. The proposed method can be useful in
the quality control of bulk manufacturing and pharmaceutical dosage forms.
Bharatkumar G. Chaudhari has completed his Ph.D. at the age of 32 years from Hemchandracharya North Gujarat University. He is an Associate
Professor in Department of Quality Assurance, S. K. Patel College of Pharmaceutical Education & Research, Ganpat University, Gujarat, India. He
has published more than 35 papers in reputed journals and serving as reviewer in various reputed journals.
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