Development Of An Analytical Method For The Determination Of Storage Lipids In Calanus Finmarchicus | 6121
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
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Development of an Analytical Method for the Determination of Storage Lipids in Calanus finmarchicus

International Conference & Exihibition On Analytical and Bioanalytical Techniques - 2010

Abdullahi Ahmed Yusuf, Lynda Webster and Patricia Pollard

ScientificTracks Abstracts: J Anal Bioanal Techniques

DOI: 10.4172/2155-9872.1000001

A method was developed for the determination of the major storage lipids, wax ester and triglycerides, in the copepod Calanus finmarchicus . A variation of the Folch method was used to extract the lipid. The method was scaled down to enable the extract ion of either pooled (~l mg) or individual (~200 μg) copepods. The major lipid classes were identified using TLC and quantified using HPLC coupled with evaporative light scattering detection. Analysis of laboratory reference materials indicated that this method underestimated the minor triglyceride component, but gave a good estimate of the major wax ester component. The fatty acid and fatty alcohol composition of the C. finmarchicus were determined following trans- esterification of the lipid extract in methanol. Fatty acids and fatty alcohols were initially identified by comparison with authentic standard and by mass spectroscopy. Using GC with flame ionisation detection the normalised area percentage of the fatty alcohols and fatty acid m ethyl esters was deter mined simultaneously in one run for either pooled or individual copepod samples. These methods were applied to C. finmarchicus collected from the Irminger Sea, North Atlantic in 2001 and 2002.