Development Of An Analytical Method For The Determination Of Storage Lipids In Calanus Finmarchicus | 6121
Journal of Analytical & Bioanalytical Techniques
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A method was developed for the determination of the major storage lipids, wax
ester and triglycerides, in the copepod
. A variation of the
Folch method was used to extract the lipid. The method was scaled down to enable
the extract ion of either pooled (~l mg) or individual (~200 μg) copepods. The major
lipid classes were identified using TLC and quantified using HPLC coupled with
evaporative light scattering detection. Analysis of laboratory reference materials
indicated that this method underestimated the minor triglyceride component,
but gave a good estimate of the major wax ester component. The fatty acid and
fatty alcohol composition of the
were determined following trans-
esterification of the lipid extract in methanol. Fatty acids and fatty alcohols were
initially identified by comparison with authentic standard and by mass spectroscopy.
Using GC with flame ionisation detection the normalised area percentage of the
fatty alcohols and fatty acid m ethyl esters was deter mined simultaneously in one
run for either pooled or individual copepod samples. These methods were applied
collected from the Irminger Sea, North Atlantic in 2001 and 2002.
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