Dipeptidyl Peptidase IV (DPP IV) Inhibitors From Plant Extracts By TLC Bioautography | 52853
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Dipeptidyl peptidase IV (DPP IV) inhibitors from plant extracts by TLC bioautography

7th International Conference and Exhibition on Analytical & Bioanalytical Techniques

Lihua Gu

Shanghai University of Traditional Chinese Medicine, China

Posters & Accepted Abstracts: J Anal Bioanal Tech

DOI: 10.4172/2155-9872.C1.025

TLC bioautography is an assay that combines chromatographic separation and in situ determination of biological activity. It has advantages over other screening methods for its ability to screen mixtures (a wide solubility range of compounds) simultaneously. In addition, it can reduce the cost and time of screening. TLC autobiography assays have been established for screening compounds for antimicrobials, antioxidants (free-radical scavengers) and enzyme inhibitors. In this presentation, the application of TLC bioautographic techniques on enzymatic reaction will be reviewed. An example of the development of a novel TLC-bioautographic method on detection of dipeptidyl peptidase IV (DPP IV) inhibitors from plant extracts will be described. The focus of the example will be on how reaction products via derivatization can facilitate such screening technology. In this case, the enzyme (DPP IV) hydrolyses substrate (Gly-Pro-p-nitroanilide) into p-nitroaniline (pNA), which diazotizes with sodium nitrite, and then reacts with N-(1-naphthyl) ethylenediamine dihydrochloride in turn to form a rose-red azo dye which provides a rose-red background on the TLC plates. The DPP IV inhibitors showed white spots on the background as they blocked enzymolysis of the substrate to produce pNA. The method was validated with respect to selectivity, sensitivity, linearity, precision, recovery, and stability after optimizing key parameters including plate type, time and temperature of incubation, concentration of substrate, enzyme and derivatization reagents, and absorption wavelength. One natural compound harmine was isolated and identified to be a potential DPP IV inhibitor in nine medicinal herbs by this method.

Lihua Gu is currently a PhD student at Shanghai University of Traditional Chinese Medicine. Her research interest includes the research and application of HPTLC, TLC-bioautographic methods and quality control standard in TCM. She has published more than 10 academic papers.

Email: [email protected]