Journal of Analytical & Bioanalytical Techniques
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The bark from deciduous tree
was used as therapeutic alkaloid
raw material. Bark was dried, milled and dissolved in to methanol overnight and
then filtered. The residue has concentrated and the proteins were precipitated with
small amount of water. After the filtration. the solution were evaporated into dryness,
The method used was similar than used for flavonoid, quercetin in pharmacognosy.
The yield consist five very similar bis-benzyl-isoquinoline alkaloids, keeping
functional control in aqua solution, so that molecule palmatine is most sensitive
to compensate the other by hydrolytic change in aqua solution. It means that
the proportion of alkaloids maintain roughly constant when isolated from natural
sources. In this schematic structure the electrons associated to nitrogen, are orphan
and most easiest to transform.
Capillary zone electropherogram over this five alkaloid mixture (theUV-200 nm �
scan) was used to monitor the changes in electrons. The movement was rocked
mildly, when alkaloid extract was solved with small amount of methanol, following
a strong excess of water by evaporating the alkaloid solution in dryness in 135
A distinct change in electropherograms was obtained. A new peak of toxic tertiary
alkaloids were changed place and formed a new dominating on the non-toxic
(tertiary) side. Simultaneously, a new pivotal function was discovered, about the
100 times improvement in therapeutic potency. The part of toxic tissue degradative
was changed and formed new pole of regenerative function.
The anastomosis is verified with liver injured laboratory rodents. Three different
lesions were selected to prove:
-GalN., ThaA and, Allyl-Alcohol. The curative
influence of anastomosis on to injured liver was observed in all cases under
studies irrespective of origin of injury. The improvement seems to follow always
same regeneration formula. In start to arrange the untidy blood flow. The special
observations was concentrated on sinusoids, which regulate the blood flow and
give impact of anastomoses (the capability to provide alternative blood flow to portal
veins) despite of obstructions, there is an increased need to transport the waste of cell
apoptosis rests and other degradation product like fibrosis of tissues, collagens and
procollagens . At same time the mitosis of hepatic cells was developed. According
to these findings the triggered anastomoses seems to be the presupposition on
successful liver remedy.
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