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Evaluation Of The Alpha-glucosidase Inhibitory Activity Of Flavonoids In The Peelings Of Citrus Dekopon [(Citrus Unshiu � C. Sinensis) � C. Reticulata] | 10221
ISSN: 2155-9872
Journal of Analytical & Bioanalytical Techniques
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Evaluation of the alpha-glucosidase inhibitory activity of flavonoids in the peelings of Citrus dekopon [(Citrus unshiu � C. Sinensis) � C. Reticulata]
4th International Conference and Exhibition on Analytical & Bioanalytical Techniques
Recently, disease such as obesity or hyperglycemia spreads by the disorder of westernization of the meal and the lifestyle in
Japan, and life-style related diseases such as metabolic syndrome or diabetes attract its attention. In particular we investigated
citrus fruit peels for diabetes in this study. We investigate here the peelings of
Citrus dekopon [(Citrus unshiu � C. sinensis) �
C. reticulata]
which is one of citrus fruits, and examined alpha-glucosidase inhibitory activity to be known as a pharmacologic
therapy of diabetes.
Crushed the peelings of
Citrus dekopon
were extracted with 70% ethanol. The 70% ethanol extract was successively treated
with 1-hexane and 1-butanol, and their extracts examined by alpha-glucosidase inhibitory activity. Remarkable inhibitory
activity was separated by gel filtration and silica gel column chromatography.
Four compounds were isolated from the peelings of
Citrus dekopon
, and their structures were established on basis of MS and
NMR spectroscopy. They were naringin (1), poncirin (2), hesperidin (3), and 2, 3- dihydroxy -3, 4?- dimethoxy flavanone 7- O
- rutinoside (4). As a result, compounds 1 and 2 were high α-glucosidase inhibitory activity. In conclusion, it might be
used in
general as food additives because remarkable alpha-glucosidase inhibitory activity was found from the peelings of
Citrus dekopon.
Biography
Hiromi Kawabata is a graduate student of Kinki University, Department of Applied Biological Chemistry. She studies isolation and bioanalytical
technique under Associate Professor Sawabe.
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