alexa Highly Sensitive Newly Designed Fluorescent Protein Probes And Application To 2D Electrophoresis | 6007
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Recommended Conferences
Share This Page

Highly Sensitive Newly Designed Fluorescent Protein Probes and Application to 2D Electrophoresis

2nd International Conference on Analytical & Bioanalytical Techniques

Kenji Yokoyama, Yoshio Suzuki, Nao Sakaguchi and Atsunori Hiratsuka

Key Note Forum: J Anal Bioanal Techniques

DOI: 10.4172/2155-9872.S1.01

Development of novel fl uorescent molecular probes with high performance is of much importance to detect proteins at high sensitivity. We designed and synthesized a fl uorescent molecule with both hydroxystyryl and cyanopyranyl moieties to detect the proteins via non-covalent bonding [1]. Th e most important and advantageous feature of this probe molecule is no fl uorescence emission in the absence of protein. Th e derivative with sulfonyl group has also been developed [2]. Th is probe is more soluble in an aqueous solution, and hence they can be applied to in-gel protein staining. In addition, we designed a more sensitive probe that is commercially available as Rapid FluoroStain KANTO (Kanto Chemical). In this study, we evaluated the performance of Rapid FluoroStain KANTO for in-gel protein staining. A fully automated two-dimensional electrophoresis (2DE) system developed by our group [3] was employed. Th is automated 2DE system can realize high-throughput proteomic analysis with high reproducibility and compact design. Sensitivity of protein staining was comparable with commercially available fl uorescent probes. It takes only 90 minutes to complete protein staining process, and this is much shorter than any other probes. In addition, we successfully designed a novel fl uorescent probe that is more sensitive than Rapid FluoroStain KANTO. We will report the details of the new probe at the conference.
Dr. Kenji Yokoyama is Deputy Director, Biotechnology Industrialization Center, National Institute of Advanced Industrial Science and Technology (AIST). He received his PhD at Tokyo Institute of Technology in 1991, and after postgraduate research at Research Center for Advanced Science and Technology, University of Tokyo, promoted to Associate Professor at School of Materials Science, Japan Advanced Institute of Science and Technology in 1994. He has moved to AIST since 2002. He has worked on electrochemical biosensors, peptide engineering and development of analytical tool for proteome