Identification And Characterization Of A Calcium Oxalate Crystal Growth Protein Inhibitor From Human Renal Stone Matrix | 6093
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Identification and Characterization of a Calcium Oxalate Crystal Growth Protein Inhibitor from Human Renal Stone Matrix

International Conference & Exihibition On Analytical and Bioanalytical Techniques - 2010

Chanderdeep Tandon

ScientificTracks Abstracts: J Anal Bioanal Techniques

DOI: 10.4172/2155-9872.1000001

A relatively small number of well-characterized inhibitors of kidney stone formation have been identified from the previous research involved in its formation. In this study conventional biochemical methods have been combined with recent advances in mass spectrometry (MS) to identify a novel calcium oxalate (CaOx) crystal growth inhibitor in human renal stone matrix. Proteins were isolated from the matrix of human CaOx containing kidney stones. Proteins having MW>10 kDa were subjected to anion exchange and molecular-sieve chromatography. Protein fractions were tested for their effects on CaOx crystal growth. Most potent fraction was excised, in- gel tryptic digested and identified by matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) MS. An anionic protein (MW~42 kDa) with potent inhibitory activity against CaOx crystal growth was purified. Its homogeneity was confirmed by RP-HPLC. It was identified by MALDI-TOF-MS followed by database search on MASCOT server as human phosphate cytidylyltransferase 1, beta. Molecular weight of this novel CaOx crystal growth inhibitor from human renal stone matrix is also the same as that of human phosphate cytidylyltransferase 1, choline, beta. Human phosphate cytidylyltransferase 1, choline, beta is a novel CaOx crystal growth inhibitor. It is involved in the biosynthesis of phosphatidylcholine which happens to be an important constituent of human renal stones and is also reported to have an antilithiatic effect.