Journal of Analytical & Bioanalytical Techniques
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Lipid homeostasis in vivo is mediated by several lipoprotein sub-species, commonly classified as high density and low density
lipoproteins (HDL and LDL). The two common apolipoproteins, ApoA-1 and ApoB-100 are associated with numerous
exchangeable lipids and proteins in various compositions. Because of their number and complexity, the analysis of lipoprotein subspecies
in plasma by bottom-up proteomics approaches requires pre-analytical physical fractionation. Asymmetric flow-field flow
fraction (AF4) is a gentle separation technique based on hydrodynamic size. AF4 allows size separation of intact lipoprotein subspecies
and fraction collection. LC-MS/MS analysis of the individual fractions can provide unique concentration versus hydrodynamic
size profiles for each protein constituent. Deconvolution of the concentration versus size profiles of ApoA-1 and ApoB-100 allows
quantitative deduction of the particle number of lipoprotein sub-species. The probability of associations of proteins with ApoA-1 and
ApoB-100 containing sub-species was evaluated based on size profile overlap with those of Apo-A-1 or ApoB-100 sub-species, size
derived maximum possible total molecular weight, and protein-ApoA-1 or protein-ApoB-100 concentration correlations measured
in multiple serum samples with wide range of cholesterol and triglyceride levels. Based on these criteria proteins can be included or
excluded as participants of ApoA-1 and ApoB-100 mediated lipid metabolism pathways.
Zsuzsanna Kuklenyik has completed her master degree in Chemical Engineering at Technical University of Budapest, and her PhD degree at Emory University of Atlanta Georgia, where she also conducted Postdoctoral studies. Currently, she is a Senior Research Scientist in the Biological Mass Spectrometry Laboratory at the Centers for Disease Control and Prevention in Atlanta. She has published more than 40 papers in reputed journals on wide range of applications of hyphenated chromatographic techniques and mass spectrometry, such as biomonitoring of environmental chemicals, analysis of pre-exposure prophylactic drugs against HIV, biological toxins, and more recently, lipoproteins.