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Implementation Of Plant Hormone Quantification In Arabidopsis Using UHPLC-MS/MS | 34630
ISSN: 2155-9872
Journal of Analytical & Bioanalytical Techniques
Open Access
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Modern plant physiology has clarified many important processes involved in plant development and crop yield. Among them,
the knowledge of the mechanisms implicated in plant response against ambient fluctuations allows us to identify interesting
genotypes. These processes have been reported to be regulated by molecules known as plant hormones. Cytokinins, gibberellins, auxins
and abscisic acid are some examples of them. They regulate physiological processes such as apical dominance, plant transpiration by
stomata closure or rooting. Although plant hormones are related to these processes, nowadays more information is needed about
their signalling and action mode in plants. In this regards, our research group is constantly working in the improvement of protocols
to analyse the variations of these molecules in different plants tissues grown under varied conditions. The wide experience in the study
of these plant growth regulators and the use of leading technologies based on ultra-high performance liquid chromatography–tandem
mass spectrometry (UHPLC-MS/MS) have permitted us to increase the number of metabolites detected as well as simplification of
purification protocol. In addition, the inclusion of micro-extraction and immuno-affinity techniques reduces the needed amount of
sample per analysis. In addition, our group has an avant-garde high throughput phenotyping to continuously monitor the relative
growth rate and to collect wide range of fluorescence parameters. The use of this facility permits us to combine and corroborate the
relationship between the plant physiological traits and the variations in hormone pathways and to identify those metabolites related
to these processes. As example, new information about the involvement between photosynthesis, carbon metabolism and changes of
cytokinin levels in Arabidopsis was found.