In Vitro Drug Interaction Assessment Between Leviteracetam And Amitriptyline By HPLC, UV DSC And Infrared Spectroscopy | 18758
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 3463

Journal of Analytical & Bioanalytical Techniques received 3463 citations as per Google Scholar report

Journal of Analytical & Bioanalytical Techniques peer review process verified at publons
Indexed In
  • CAS Source Index (CASSI)
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Academic Journals Database
  • Open J Gate
  • Genamics JournalSeek
  • JournalTOCs
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Ulrich's Periodicals Directory
  • Electronic Journals Library
  • RefSeek
  • Directory of Research Journal Indexing (DRJI)
  • Hamdard University
  • OCLC- WorldCat
  • Scholarsteer
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Euro Pub
Share This Page

In vitro drug interaction assessment between leviteracetam and amitriptyline by HPLC, UV DSC and infrared spectroscopy

5th International Conference and Exhibition on Analytical & Bioanalytical Techniques

J C Thejaswini and B M Gurupadayya

Accepted Abstracts: J Anal Bioanal Tech

DOI: 10.4172/2155-9872.S1.019

In present study, it is described an in vitro drug interaction assessment of prophylactic agents used in treatment of migraine; leviteracetam and amitriptyline. Several drugs even though not marketed as single dosage form are co-administered leads to drug interactions. Leviteracetam is an antiepileptic drug and amitriptyline is a tricyclic antidepressant, are used as prophylactic drugs in treatment of migraine. As per literature survey, no analytical methods are available for their simultaneous analysis. In present study, a simple RP-HPLC and UV method for simultaneous determination of leviteracetam and amitriptyline has been developed. For HPLC method, a mixture of acetonitrile, methanol and phosphate buffer of pH 3 in the ratio 40:40:20 was used as mobile phase. Elution was carried out in C18 column and detected using PDA detector. The developed method was validated following ICH guidelines. In vitro dissolution study was performed by HPLC and UV methods in order to assess the influence of one drug on the dissolution behavior of other drug in simulated intestinal juice and gastric juice. Aliquots were withdrawn periodically with an interval of 15 min from 0 to 180 min and their concentrations were determined by RP- HPLC and UV spectroscopy and the results were compared. Remarkable decrease in absorption was observed when two drugs were simultaneously introduced for dissolution studies compared to their absorbance as individual drugs. HPLC results also show the antagonistic effect of one drug on the dissolution pattern of the other. It was also performed the IR, DSC interaction studies in order to check the changes in the significant peaks due to drug interactions. By both the techniques changes in the peaks were observed indicating the structural modification of the drugs which may be the factor for changes in the dissolution behavior of combination of drugs.