In Vitro Drug Interaction Assessment Between Leviteracetam And Amitriptyline By HPLC, UV DSC And Infrared Spectroscopy | 18758
Journal of Analytical & Bioanalytical Techniques
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In present study, it is described an
drug interaction assessment of prophylactic agents used in treatment of migraine;
leviteracetam and amitriptyline. Several drugs even though not marketed as single dosage form are co-administered leads to
drug interactions. Leviteracetam is an antiepileptic drug and amitriptyline is a tricyclic antidepressant, are used as prophylactic
drugs in treatment of migraine. As per literature survey, no analytical methods are available for their simultaneous analysis.
In present study, a simple RP-HPLC and UV method for simultaneous determination of leviteracetam and amitriptyline has
been developed. For HPLC method, a mixture of acetonitrile, methanol and phosphate buffer of pH 3 in the ratio 40:40:20
was used as mobile phase. Elution was carried out in C18 column and detected using PDA detector. The developed method
was validated following ICH guidelines.
dissolution study was performed by HPLC and UV methods in order to assess
the influence of one drug on the dissolution behavior of other drug in simulated intestinal juice and gastric juice. Aliquots
were withdrawn periodically with an interval of 15 min from 0 to 180 min and their concentrations were determined by RP-
HPLC and UV spectroscopy and the results were compared. Remarkable decrease in absorption was observed when two drugs
were simultaneously introduced for dissolution studies compared to their absorbance as individual drugs. HPLC results also
show the antagonistic effect of one drug on the dissolution pattern of the other. It was also performed the IR, DSC interaction
studies in order to check the changes in the significant peaks due to drug interactions. By both the techniques changes in the
peaks were observed indicating the structural modification of the drugs which may be the factor for changes in the dissolution
behavior of combination of drugs.
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