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In Vivo And In Vitro Analysis By Raman-SNOM-AFM Imaging And Femtosecond Spectroscopy –from Single Cells To Humans. Raman Diagnostic Markers For Breast Cancer Prognosis. New Look Inside Human Breast Ducts With Raman Imaging | 34624
ISSN: 2155-9872
Journal of Analytical & Bioanalytical Techniques
Open Access
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In vivo and in vitro analysis by Raman-SNOM-AFM imaging and femtosecond spectroscopy –from single cells to humans. Raman diagnostic markers for breast cancer prognosis. New look inside human breast ducts with Raman imaging
6th International Conference and Exhibition on Analytical & Bioanalytical Techniques
Halina Abramczyk, Beata Brozek-Pluska and Jakub Surmacki
This contribution will explore cutting edge molecular (Raman, fluorescence, SNOM, AFM, TERS, femtosecond spectroscopy)
mapping and time resolved dynamics of cellular structures of cancers, localization of drugs and nanoparticles in cells and tissues.
The multidisciplinary nature of the studies span a diverse range of biological, chemical, and physical sciences related to cancer
biology. This contribution will provide insight regarding Raman Hyperspectral Microscopy technique and its ability to enable rapid
optical observation and spectral confirmation of unlabeled biomolecules, drugs, and nanomaterials as they interact with cells and
tissue. The main focus will be on the integration of near field microscopy, AFM and hyperspectral Raman imaging technology to
look inside human breast ducts to give unambiguous answer about location and distribution of biochemical components in human
cells and tissue during cancer development. The contribution shows new look inside human breast duct using Raman imaging, an
emerging technology of molecular imaging, that may bring revolution in understanding of cancer biology. Our contribution is a
first report in the literature demonstrating such a detailed analysis of normal and cancerous ducts in human breast tissue. The main
advantage of Raman imaging is that it gives spatial information about various chemical constituents in defined cellular organelles in
contrast to conventional methods (LC/MS, NMR, and HPLC) that rely on bulk or fractionated analyses of extracted components.