Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
Inhibitors Of Caspases As New Therapeutic Agents | 6050
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 3732

Journal of Analytical & Bioanalytical Techniques received 3732 citations as per Google Scholar report

Journal of Analytical & Bioanalytical Techniques peer review process verified at publons
Indexed In
  • CAS Source Index (CASSI)
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Academic Journals Database
  • Open J Gate
  • Genamics JournalSeek
  • JournalTOCs
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Ulrich's Periodicals Directory
  • Electronic Journals Library
  • RefSeek
  • Directory of Research Journal Indexing (DRJI)
  • Hamdard University
  • OCLC- WorldCat
  • Scholarsteer
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Euro Pub
Share This Page

Inhibitors of Caspases as New Therapeutic Agents

International Conference & Exihibition On Analytical and Bioanalytical Techniques - 2010

N.Mallikarjuna Rao

ScientificTracks Abstracts: J Anal Bioanal Techniques

DOI: 10.4172/2155-9872.1000001

Caspases are diverse group of enzymes involved in apoptosis and inflammation. Activation of caspases contributes to large number of pathological conditions a) Apoptic disorders such as myocardial infarction, lung diseases, liver diseases and neuronal diseases b) Inflammatory disorders such as rheumatoid arthritis, osteoarthritis, gout and psoriasis. Inhibition of caspase activity is therapeutically effective in these disorders. Active site structure based approach, Structure activity relationship (SAR) based approach, peptidomimetics, high trough put screens (HTS) and fragment based approach teethering are currently used to discover caspase inhibitors. Caspases are cysteine proteases containing cysteine at active site and cleaves-D(Asp)-X-bonds. Active site cysteine and nearby histidine forms catalytic diad. Binding of negatively charged aspartic acid moiety of the substrate is favoured by positively charged arginine and glutamine. Catalysis of peptide bond cleavage by caspase involves formation of tetrahedral transition state thio hemi katal initiated by active site cysteine acting as nucleophile. Near by histidine residue aids product release. Inhibition by reversible inhibitors involves formation of thio hemi katal similar to tetrahedral transition state analog of substrate. A reversible caspase inhibitor pralancasan (VX-740) is discovered by active site directed approach. Clinical trials show that pralancasan is an effective anti inflammatory drug. Irreversible caspase inhibitors forms thio ether adduct resulting in inactivation of enzyme. An irreversible peptidomimetic inhibitor emricasan (1DN-6556) is designed from di peptide backbone which is effective in apoptic driven disorders. Isatins, a group of small molecule inhibitors are identified by high through put screens. Isatins contains a carbonyl group which is critical for inhibitory activity. Caspases are inactivated due to interaction of carbonyl group of isatins with active site Cysteine.