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Method Development And Validation For Simultaneous Determination Of Sitagliptin Phosphate And Metformin Hydrochloride By RP-HPLC In Bulk And Tablet Dosage Form | 5883
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
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Method development and validation for simultaneous determination of sitagliptin phosphate and metformin hydrochloride by RP-HPLC in bulk and tablet dosage form

3rd International Conference and Exhibition on Analytical & Bioanalytical Techniques

A.B. Loni

Posters: J Anal Bioanal Techniques

DOI: 10.4172/2155-9872.S1.010

The pyrazole scaffold represents a common motif in many pharmaceutical active and remarkable compounds demonstrating a wide range of pharmacological activities; the most important activities are the anti-inflammatory, the antibacterialantifungal, the hypoglycemic, the anti-hyperlipidemic, the inhibition of cyclooxigenase-2, p38 MAP kinase and CDK2/Cyclin A, and the antiangiogenic. In the present work we are aiming to synthesis novel pyrazole derivatives together with their antitumor evaluations. the reaction of cyanoacetylhydrazine with chloroacetylchloride gave the N-acyl derivative 3. The the latter underwent ready cyclization in sodium ethoxide to give the pyrazole derivative 4 which was the key compound for the synthesis of thiophene, thieno[2,3-b]pridine and thiazole derivatives. The cytotoxicity of the newly synthesized products towards against three human tumor cells lines namely breast adenocarcinoma (MCF 7), non-small cell lung cancer (NCI-H460) and CNS cancer (SF-268) and some of these compounds were found to exhibit much higher inhibitory effects towards the three tumor cell lines than the reference doxorubicin.
Rafat Mohareb has completed his Ph. D at the age of 26 years from Cairo University. He got full Professer in 1994, participating with International fellows foundations like: the Alexander von Humboldt foundation, Full bright and the Arab-fund Foundation for Social and economic developments. He was published around 125 papers in the field of Pharmaceutical Chemistry.