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Mixed-mode High-performance Liquid Chromatography And Its Potential Use In Pharmaceutical Analysis | 10296
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
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Mixed-mode high-performance liquid chromatography and its potential use in pharmaceutical analysis

4th International Conference and Exhibition on Analytical & Bioanalytical Techniques

Pavel N. Nesterenko

Accepted Abstracts: J Anal Bioanal Tech

DOI: 10.4172/2155-9872.S1.015

For the last decades HPLC remained the major analytical method in pharmaceutical analysis. The current trend in the development of HPLC is about simultaneous analysis of multi-component formulations comprising very different substances. As a rule a complete chromatographic analysis can be accomplished by using: i) separate HPLC modes/instruments equipped with columns having different selectivity; ii) multi-dimensional chromatography and iii) mixed-mode chromatography. The latter approach combines the advantages of simplicity, robustness and economic factors. Mixed-mode liquid chromatography has been at the forefront of separation techniques in the recent years offering superior selectivity, resolution and efficiency. The current lecture will critically evaluate a number of individual modes in liquid chromatography namely reversed phase, normal phase, ion-exchange, chromatography of hydrophilic interactions and others followed by their synergetic effects to provide mixed-mode retention mechanism in the analysis of small molecules. The key role in mixed mode chromatography belongs to the selectivity of column packing, so a special attention will be paid to the classification of chromatographic columns and adsorbents, which can provide several retention mechanisms with one type of mobile phase. The methods for the comparison and for characterization of separation selectivity of mixed-mode stationary phases will be also considered, as well as possible applications.
Pavel N. Nesterenko received Ph.D. (1984) and D.Sc (2000) degrees in Lomonosov Moscow State University. At present, he holds a position of New Stars Professor at the University of Tasmania, Hobart, Australia. Adjunct professor of Dublin City University, Ireland since 2005, Visiting Honorary Research Fellow of the University of Plymouth, UK since 2003. He is the author of 250 scientific publications including 2 monographs, 7 Chapters in books, 20 reviews and 220 regular papers. He is the member of advisory and editorial boards for 6 international journals in the field of analytical chemistry and separation sciences