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Mixed-mode High-performance Liquid Chromatography And Its Potential Use In Pharmaceutical Analysis | 10296
Journal of Analytical & Bioanalytical Techniques
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For the last decades HPLC remained the major analytical method in pharmaceutical analysis. The current trend in the
development of HPLC is about simultaneous analysis of multi-component formulations comprising very different substances.
As a rule a complete chromatographic analysis can be accomplished by using: i) separate HPLC modes/instruments equipped
with columns having different selectivity; ii) multi-dimensional chromatography and iii) mixed-mode chromatography. The
latter approach combines the advantages of simplicity, robustness and economic factors.
Mixed-mode liquid chromatography has been at the forefront of separation techniques in the recent years offering
superior selectivity, resolution and efficiency. The current lecture will critically evaluate a number of individual modes in liquid
chromatography namely reversed phase, normal phase, ion-exchange, chromatography of hydrophilic interactions and others
followed by their synergetic effects to provide mixed-mode retention mechanism in the analysis of small molecules. The key role
in mixed mode chromatography belongs to the selectivity of column packing, so a special attention will be paid to the classification
of chromatographic columns and adsorbents, which can provide several retention mechanisms with one type of mobile phase.
The methods for the comparison and for characterization of separation selectivity of mixed-mode stationary phases will be also
considered, as well as possible applications.
Pavel N. Nesterenko received Ph.D. (1984) and D.Sc (2000) degrees in Lomonosov Moscow State University. At present, he holds a position of New
Stars Professor at the University of Tasmania, Hobart, Australia. Adjunct professor of Dublin City University, Ireland since 2005, Visiting Honorary
Research Fellow of the University of Plymouth, UK since 2003. He is the author of 250 scientific publications including 2 monographs, 7 Chapters
in books, 20 reviews and 220 regular papers. He is the member of advisory and editorial boards for 6 international journals in the field of analytical
chemistry and separation sciences
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