alexa
Reach Us +44-2476101207
M-protein Binding Peptides From Phage Display Libraries As Biomarkers In Multiple Myeloma: A Paradigm For Early Detection Of Disease Relapse | 6086
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

M-protein Binding Peptides from Phage Display Libraries as Biomarkers in Multiple Myeloma: a Paradigm for Early Detection of Disease Relapse

International Conference & Exihibition On Analytical and Bioanalytical Techniques - 2010

Michael A. Firer

ScientificTracks Abstracts: J Anal Bioanal Techniques

DOI: 10.4172/2155-9872.1000001

Abstract
The detection of specific biomarkers with simple laboratory tests can may important implications in the diagnosis, treatment and survival of patients. An example is Multiple Myeloma (MM), an incurable B-cell leukemia. With the use of new drugs, most MM patients now enter into a period of remission; however they inevitably relapse. MM tumor cells derive from a single clone of plasma cells that usually express and secrete excess amounts of clonotypic immunoglobulin (M-protein). Within each patient, M-protein V H gene sequences differ suggesting that these antibodies are directed against different antigens. Currently, electrophoresis and gel immunofixation are used to identify the presence and isotype of M-proteins in serum, however these methods are both tedious and insensitive. Particularly with regards to early intervention in patients in remission, a more sensitive method that heralds the presence of the specific M-protein biomarker would indicate disease relapse, leading to earlier resumption of treatment and potentially enhanced patient survival. To this end, we have used phage display peptide libraries to isolate peptides that bind M-proteins from MM patients. Bioinformatic analyses of the peptide sequences determined their homology to natural proteins of clinical significance including proteins from bacterial species associated with respiratory infections and food poisoning. These peptides can then be conjugated to fluorescent or other reporter molecules and used in simple immunoassays to follow the reappearance of the patient's M-protein in serum. The isolation of biomarker-binding peptides and their use in sensitive immunoassays is a platform approach that can be applied to development of improved methods for the monitoring of patients.
Biography
Top