Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar


New Approaches For The Synthesis Of Pharmaceutically Active Heterocyclic Compounds | 5977
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Recommended Conferences

3rd International Conference on Analytical Chemistry

Prague, Czech Republic
Google Scholar citation report
Citations : 4454

Journal of Analytical & Bioanalytical Techniques received 4454 citations as per Google Scholar report

Journal of Analytical & Bioanalytical Techniques peer review process verified at publons
Indexed In
  • CAS Source Index (CASSI)
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Academic Journals Database
  • Open J Gate
  • Genamics JournalSeek
  • JournalTOCs
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Ulrich's Periodicals Directory
  • Electronic Journals Library
  • RefSeek
  • Directory of Research Journal Indexing (DRJI)
  • Hamdard University
  • OCLC- WorldCat
  • Scholarsteer
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Euro Pub
Share This Page

New approaches for the synthesis of pharmaceutically active heterocyclic compounds

3rd International Conference and Exhibition on Analytical & Bioanalytical Techniques

Rafat M. Mohareb

ScientificTracks Abstracts: J Anal Bioanal Techniques

DOI: 10.4172/2155-9872.S1.007

Introduction: Streptococcus pyogenes (Group A Streptococcus, GAS) is the causative agent of many human diseases, including pharyngitis, rheumatic fever and necrotizing fasciitis. Recent studies indicate Streptococci produce and respond to several secreted signaling peptides that are imported into the cell and subsequently bind to regulator proteins of the Rgg family. In GAS, four Rgg paralogs exist, each serving as transcriptional regulators of genes associated with pathogenesis (RopB), biofilm induction (Rgg2 and Rgg3), or a cryptic competence regulon (ComR). The genes encoding peptide pheromones for modulating the activity of Rgg2, Rgg3 and ComR are known, while the one leading to RopB activation remains unknown. The aim of this study is to identify and elucidate the precursor and active peptide pheromones from culture supernatants that control each Rgg pathway. Methods: Spent culture media was fractionated using low-molecular weight filters and reverse phase chromatography steps to isolate peptide pheromones. The activity of HPLC fractions was tested by luciferase reporters that were generated by fusing the luxAB genes to promoters controlled by each Rgg protein. Mass spectrometry was used to identify active molecules. Results and Conclusions: We demonstrate that GAS spent culture supernatants contain at least four specific factors that can induce independent transcriptional reporters. Mass-spectrometry analysis is underway to elucidate each active pheromone. Revelation of the mature peptide pheromones will be a first step in designing antagonistic peptides used for future therapeutics designed to interfere with intercellular signaling in GAS and in other Rgg-containing microbes.

Chaitanya Aggarwal did his B.Tech in Biotechnology from Kurukshetra University, Kurukshetra in 2008. He then worked in Biologics Development Unit of Dr. Reddy?s labs at Hyderabad for one year. He moved to USA in 2009 to pursue PhD at University of Illinois, Chicago in Center for Pharmaceutical Biotechnology. His PhD research, under the guidance of Dr. Michael Federle, focuses on understanding bacterial cell-to-cell communication, termed as quorum-sensing, in human pathogen Streptococcus pyogenes.