alexa

GET THE APP

Nucleic Acid Biosensor For Detection Of Salmonella Typhimurium Detection | 10268
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google scholar citation report
Citations : 3463

Journal of Analytical & Bioanalytical Techniques received 3463 citations as per google scholar report

Journal of Analytical & Bioanalytical Techniques peer review process verified at publons
Indexed In
  • CAS Source Index (CASSI)
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Academic Journals Database
  • Open J Gate
  • Genamics JournalSeek
  • JournalTOCs
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Ulrich's Periodicals Directory
  • Electronic Journals Library
  • RefSeek
  • Directory of Research Journal Indexing (DRJI)
  • Hamdard University
  • EBSCO A-Z
  • OCLC- WorldCat
  • Scholarsteer
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Euro Pub
Share This Page

Nucleic acid biosensor for detection of Salmonella typhimurium detection

4th International Conference and Exhibition on Analytical & Bioanalytical Techniques

Nirmal Prabhakar and Hemkusha Thakur

Accepted Abstracts: J Anal Bioanal Tech

DOI: 10.4172/2155-9872.S1.015

Abstract
We report here the development of electrochemical DNA hybridization biosensor for detection of Salmonella typhimurium. Two types of DNA probes (biotin labeled and amine labeled) specific to S. typhimurium have been immobilized onto the carbon nanotube-polyaniline composite films (CNT-PANI) coated onto indium tin oxide (ITO) glass plates. The fabricated bioelectrodes (biotin-DNA/CNT-PANI/ITO electrode and NH 2 -DNA/CNT-PANI/ITO electrodes) have been characterized using electrochemical techniques, fourier-transform-infra red spectroscopy (FT-IR) and scanning electron microscopy (SEM). These Biotin-DNA/CNT-PANI/ITO electrode and NH 2 -DNA/CNT-PANI/ITO electrodes have been utilized to detect hybridization with complementary, non-complementary and one-base mismatch sequences using methylene blue (MB) as electroactive indicator. Results indicate that the NH2-DNA/CNT-PANI/ITO electrode has better detection limit (0.1 attomole) over the biotin- DNA-CNT-PANI/ITO electrode (0.5 attomole) within 30 seconds as compared to 35 seconds for biotin-DNA-CNT-PANI/ITO electrode. The NH 2 -DNA/CNT-PANI/ITO electrode has the capability to detect the hybridization with complementary sequences in genomic DNA of S. typhimurium within 60 seconds.
Biography
holiganbetperabetimajbettipobetVdcasino
Top