Ocular Pharmacokinetics Of Antiviral Nanoformulations By Ultra High-pressure Liquid Chromatographic Method | 6056
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Ocular Pharmacokinetics of Antiviral Nanoformulations by Ultra High-pressure Liquid Chromatographic Method

International Conference & Exihibition On Analytical and Bioanalytical Techniques - 2010

Sohail Akhter, Gaurav K Jain, Shadab A Pathan, Musarrat H. Warsi, Prakash Chander, Sushma Talegaonkar, Roop K Khar and Farhan J Ahmad

ScientificTracks Abstracts: J Anal Bioanal Techniques

DOI: 10.4172/2155-9872.1000001

The present work describes a rapid and sensitive advance liquid chromatographic technique, ultra high-pressure liquid chromatography (UHPLC) method with UV detection to quantify antiviral drug ganciclovir (GCV) in rabbit aqueous humor. After deproteinisation with acetonitrile, gradient separation of GCV was achieved on a Waters Acquity BEH C18 (50 mm x 2.1 mm, 1.7 μm) column at 50�C. The mobile phase consisted of 0.1% trifluoroacetic acid in water (pH 3.5) and acetonitrile (95:5, v/v) at a flow rate of 0.45 mL/min. GCV analysis was performed at a wavelength of 254 nm with total run time of 3 min. Method was found to be selective, linear ( r 2 = 0.999), accurate (recovery, 97.0�100.2%) and precise (CV, ≤ 3.1%) in the selected concentration range of 0.1�1.0 μg/mL. Detection and quantitation limit of GCV in aqueous humor were 3.0 and 10.0 ng/mL, respectively. The method was applied to compare aqueous humor levels of GCV after single topical instillation of GCV solution, GCV nanoparticles, GCV nanocomplexes and GCV niosomal dispersions. Topical instillation of GCV-NCs (AUC 0→t , 3440.7�26.2 and GCV-NDs (AUC 0→t , 3380.5�29.3 provided approximately 5 fold increase in the relative ocular bioavailability compared with GCV solution (AUC 0→t , 650.8�14.9 ng.h/ mL) and nearly 2.5 fold higher than the GCV-NPs (AUC 0→t , 1350.2�18.5 ng.h/mL). The results indicate that the nanocomplexes and niosomal dispersions increases ocular bioavailability of GCV and prolong its residence time in the eye.