Performance Of Spectral And Imaging Technologies | 5981
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
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Performance of spectral and imaging technologies

3rd International Conference and Exhibition on Analytical & Bioanalytical Techniques

N. B. Singh

AcceptedAbstracts: J Anal Bioanal Techniques

DOI: 10.4172/2155-9872.S1.011

A novel gas liquid chromatographic (GC) method has been developed for the quantitative estimation of Sitagliptin in bulk drug and pharmaceutical dosage forms. Ethyl chloroformate (ECF) was used as a precolumn derivatizing reagent. GC separation was carried out on an Rtx-5 capillary column (cross bond 5% diphenyl/ 95% dimethyl polysiloxane) with a length of 30 meters and an internal diameter of 0.25 mm with flame ionization detector. The elution was carried out at an initial temperature of 80? C for 4 minutes and temperature increased at the rate of 100?C/min up to 180?C for 5 min. Column pressure was programmed as 29.8 Kpa for 3.5 minutes and pressure increased at rate of 20Kpa/min up to 120Kpa for 6 minutes. The linear calibration ranges for LEV was observed between 2- 10 ng/ml. The method was subsequently applied to the determination of LEV in pharmaceutical preparations. The relative standard deviation (RSD) was found to be 0.27%. The recovery studies were done and the percentage recovery of LEV was found to be 98.36%
Sruthi is a student of JSS College of Pharmacy, JSS University, Mysore. She has completed her B.Pharm from Raghu College of Pharmacy, Vishakhapatnam. Presently she is pursuing M.Pharm Degree in the branch of Pharmaceutical analysis. Her current area of research is on analytical method development of novel drugs using HPLC and GC.