Journal of Analytical & Bioanalytical Techniques
Open Access

Abstract

Cancer is a group of diseases that arise from uncontrolled growth, spread of an abnormal cell and can result in death. The inefficiency (Tong et al , 1995) to treat several distinct classes of tumours led researchers to source for potential natural-based therapeutic compounds. Many Botanists (Etta, 2008) believed that the extract of P. niruri, (30 - 40 cm in height) originated from India by late of 1980s showed pharmacological, clinical efficacy against viral Hepatitis B (Padma et al ., 1999; Paranjpe, 2001; Blumberg et al ., 1990; Venkateswaran et al ., 1987) anti- bacterial activity (Mazumder et al ., 2006); Kloucek et al ., 2005), anti-hepatotoxic or liver-protecting activity (Houghton et al ., 1996; Rajeshkumar et al ., 2000; Jeena et al., 1999), as well as anti-tumor and anti-carcinogenic properties (Rajeshkumar et al ., 2001). In addition, it also exhibits hypoglycaemia properties (Mazunder et al ., 2005; Raphael et al ., 2002). The objective of the present study determines the cytotoxic effect of Phyllanthus extracts (aqueous and methanol) on growth inhibition against skin melanoma and prostate cancer cells in their cell cycle could partially explain its mode of activity and proliferation effect with apoptosis induction and cell cycle modulation. From the results, Phyllanthus plant appears to possess antiproliferative (cytotoxic) properties against breast, lung, melanoma and prostate cancer cells with IC 50 values ranging between 150�400 �?¼g/ml for the aqueous extract and 50�150 �?¼g/ml for the metha - nolic extract which were determined using the MTS reduction assay. In compari - son, the plant extracts did not show significant cytotoxicity on normal human cells (breast, lung, skin (CRL-2565) and prostate (RWPE-1) cells). This indicates that Phyllanthus is one step closer to being a suitable candidate for the development of effective anticancer drugs.

Biography