Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
GET THE APP
Preparation Of Anti-human IgG-hrp Conjugate And Its Use In ELISA And Western Blotting Experiments | 5891
ISSN: 2155-9872
Journal of Analytical & Bioanalytical Techniques
Open Access
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
A highly sensitive method for detection and quantification of swertiamarin, mangiferin and amarogentin in Swertiachirata
(Gentianaceae).The leaves, roots, stem, flowers and tissue cultured plants were extracted with 80% methanol in a bath
sonication for rapid extraction of metabolites. The desired separation and baseline was obtained in a gradient method where
mobile phase A was composed of 0.1% TFA in water and mobile phase B was a mixture of acetonitrile: water in the ratio of 70:30.
The linear gradient at a flow rate of 1.0ml/min was start with 15% B; 20% B in next 5 min, 70% B in next 25 min, hold for 5 min;
15%B in next 5 and equilibrated for 5 min at 240 nm UV wavelength. The separation was obtained on Waters Spherisorb 5μm
ODS2 (250 mm x 4.6 mm). The LOD of swertiamarin, mangiferin and amarogentin were found to be 0.4μg/ml, 0.2μg/ml and
0.3μg/ml respectively. The LOQ of swertiamarin, mangiferin and amarogentinwere found to be 1.0μg/ml, 0.4μg/ml and 0.9μg/
ml respectively. The method can be used as quality control of plant material and also identification of
Swertiachirata
from other
species of
Swertia
due to the presence of marker compound amarogentin.
Biography
Varun Kumar have completed his M. Pharmacy from GJU, Hissar and is having 2 years of Research experience in Analytical research and
development, Jubilant Life Sciences. Now pursuing PhD in Jaypee University of Information Technology, Shimla Hills.
Relevant Topics
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals