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Preparation Of Quality Control Charts In The Field Of Pharmaceuticals | 5986
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
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Preparation of quality control charts in the field of pharmaceuticals

3rd International Conference and Exhibition on Analytical & Bioanalytical Techniques

Thadkapally Srinivas, Goli Naresh Kumar, Aramalla Eashwar rao and Arrem Divya sree

AcceptedAbstracts: J Anal Bioanal Techniques

DOI: 10.4172/2155-9872.S1.011

Phenylephrine hydrochloride (PHEN), Paracetamol (PARA), and Caffeine (CAF) are frequently associated in pharmaceutical formulations against the common cold. A simple, precise and robust High Performance Thin Layer Chromatography method for the simultaneous quantification of PHEN, PARA and CAF in tablet was developed and validated. The method employed three drugs on Merck Aluminium based silica gel 60 F 254 plates as stationary phase and Methylene chloride: Ethyl acetate: Ethanol: Formic acid (3.5:2:4:0.5v/v) as mobile phase. Spectrodensitometric scanning was performed at 220 nm. The developed method gave well resolved spots for Phenylephrine hydrochloride (R f 0.31 ? 0.01), Paracetamol (R f 0.63 ? 0.01) and Caffeine (R f 0.81 ? 0.02). The developed method was validated for linearity, accuracy, precision, robustness and specificity studies as per the ICH guidelines. It was found to be linear over concentration ranges of 10-70 ng/μl, 150-1050 ng/μl and 50-350 ng/μl for PHEN, PARA and CAF respectively. The limit of detection and limit of quantification values were found to be 2.32 ng/μl, 10.2 ng/μl and 8.2 ng/ μl; and 7.72 ng/μl, 33.96 ng/μl and 27.97 ng/μl for, PHEN, PARA and CAF respectively. The accuracy of the developed method was estimated by performing recovery studies. The method enabled average recoveries of 97.93 % for PHEN, 98.96 % for PARA and 98.05 % for CAF. No chromatographic interference from the tablet excipients was seen in this method. The method proved to be simple and rapid for routine simultaneous estimation of Phenylephrine HCl, Paracetamol and Caffeine in the bulk drug and in a tablet formulation.
Gouri Gajanan Joshi has completed her B. Pharm from S. R. T. M. University, Nanded and now purusing M. Pharm course in Pharmaceutical Chemistry Department, from Pune University. She has participated in the various state and college level poster presentation contests at B. Pharm and M. Pharm level. She has been also the part of the various cultural activities held at the college level.