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Quantitation Of Donepezil And Its Active Metabolite 6-O-desmethyl Donepezil In Human Plasma By A Selective And Sensitive Liquid Chromatographytandem Mass Spectrometric Method | 6000
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
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Quantitation of donepezil and its active metabolite 6-O-desmethyl donepezil in human plasma by a selective and sensitive liquid chromatographytandem mass spectrometric method

2nd International Conference on Analytical & Bioanalytical Techniques

Bhavinkumar N. Patel

Accepted Abstracts: J Anal Bioanal Techniques

DOI: 10.4172/2155-9872.S1.05

A sensitive and selective LC-MS/MS assay for the simultaneous determination of donepezil (D) and its pharmacologically active metabolite, 6- O -desmethyl donepezil (ODD) in human plasma is developed using galantamine as internal standard (IS). Th e analytes and IS were extracted from 500 μL aliquots of human plasma via solid phase extraction on Waters Oasis HLB cartridges. Chromatographic separation was achieved in a run time of 6.0 min on a Waters Novapak C18 (150 mm x 3.9 mm, 4 μm) column under isocratic conditions. Detection of analytes and IS was done by tandem mass spectrometry, operating in positive ion and multiple reaction monitoring (MRM) acquisition mode. Th e protonated precursor to product ion transitions monitored for D, ODD and IS were at m/z 380.1 → 91.2, 366.3 → 91.3 and 288.2 → 213.2 respectively. Th e method was fully validated for its selectivity, interference check, sensitivity, linearity, precision and accuracy, recovery, matrix eff ect, ion suppression/enhancement, cross specifi city, stability and dilution integrity. A linear dynamic range of 0.10-50.0 ng mL -1 for D and 0.02-10.0 ng mL -1 for ODD was evaluated with mean correlation coeffi cient (r) of 0.9975 and 0.9985 respectively. Th e intra-batch and inter-batch precision (%CV) across fi ve validation runs (LLOQ, LQC, MQC, HQC and ULOQ) was less than 7.5 % for both the analytes. Th e method was successfully applied to a bioequivalence study of 10 mg donepezil tablet formulation in 24 healthy Indian male subjects under fasting condition.
Dr. Bhavin kumar patel has completed his PhD at the age of 31 from School of science, Gujarat University, is currently working as a Senior Research Scientist-I (project manager) in BA Research India Ltd. Authored Eight Scienti fi c Papers in Reputed International Journals along with Mr. Naveen Sharma, Dr. (Mrs.) Mallika Sanyal and Dr. Pranav S. Shrivastav.