Separation Of Peptides And Proteins In Tryptic Digest Of Cytochrome C By Novel Step Elution Approach In Open Tubular Capillary Electrochromatography | 52835
Journal of Analytical & Bioanalytical Techniques
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A multi-monomer based copolymer layer was immobilized on the inner surface of a pretreated 1.1 m long silica capillary
column (50 μm internal diameter and 1.02 m effective length) after the attachment of 4-(trifluoromethoxy)phenyl
isocayanate and sodium diethyl dithiocarbamate initiator system. The attachment of initiator system to silanol functionalities
on the inner capillary surface was assisted by dibutyl tin dichloride catalyst. The copolymer immobilized open tubular
capillary column resulted in the separation of about 40 peaks out of tryptic digest of cytochrome C sample in capillary
electrochromatography with high separation efficiency (Ca. 220,000 plates/column) for some of the peptide peaks. A novel
step elution approach was also demonstrated for the separation of tryptic digest of cytochrome C where two mobile phases
having different water content were used during the same run resulting in the separation of higher number of peptide peaks
(Ca. above 50) out of tryptic digest of cytochrome C, with much improved peak capacity. The step elution approach in addition
to the open tubular nature and increased column length could be a good strategy for proteomic analysis with enhanced peak
capacity in capillary electro-chromatography.
Faiz Ali has completed his PhD at the age of 31 years from INHA University South Korea and postdoctoral studies from the same University. He is the Editorial board member for the UK Journalof Pharmaceutical and Biosciences (UKJPB)http://www.ukjpb.com/editorial_board.html. He has published about 12 papers in reputed SCI journals and has presented about 12 conference presentation in international reputed conferences.