Journal of Analytical & Bioanalytical Techniques
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A simple, sensitive and specific liquid chromatographic method with UV detection (230 nm) was developed for the simultaneous
estimation of Metformine, Pioglitazone and Glibenclamide in tablet dosage form and Gliclazide as an internal standard.
Separation was achieved with an phenomenex luna 5μ CN 100R, 250X4.60 mm 5 micron size column, ambient temprature with
a low pressure gradient mode with mobile phase containing acetonitril, water and 0.5% of potassium dihydrogen phosphate
buffer pH 2.5 adjusted with orthophosphoric acid (60:20:20). The flow rate was 1 mL min
and eluent was monitored at 230 nm.
The selected chromatographic conditions were found to effectively separate Metformine, Pioglitazone and glibenclamide with
retention time of 2.2, 2.8 and 5.8 min respectively. The linearity range of Metformine, Pioglitazone and Glibenclamide is found
in the range of 50-300 μgml
, 1.5-9.0 μgml
and 0.5-3.0 μgml
respectively. The proposed method was found to be accurate,
precise, reproducible and specific and it can also be used for routine quality control analysis of these drugs in combination tablets.
Anand Kumar is purusing his PhD in JSS University, JSS College of Pharmacy Mysore, under the guidance of Dr. B. M. Gurupadayya, Professor Department of Pharmaceutical Chemistry, JSS College of Pharmacy, Mysore. He has published more than 40 papers in reputed journals.
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